PCR法进行胚胎性别鉴定的研究进展  

Sex Identification in Embryos with Polymerase Chain Reaction

作　　者：王晗[1] 窦忠英[1] 

机构地区：[1]西北农林科技大学国家干细胞工程技术研究中心陕西分中心,陕西杨凌712100

出　　处：《西北农业学报》2005年第2期5-8,共4页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家自然科学基金(30070374);陕西省重点攻关项目。

摘　　要：通过胚胎性别鉴定,可控制家畜后代性别比例,获得巨大经济效益。早期胚胎性别鉴定的方法有,核型分析法,H-Y抗原法,X-连接酶检测法和PCR扩增法等。PCR法因其简单、快速、准确等优点,成为目前比较理想的胚胎性别鉴定方法。笔者对性别决定基因、PCR法胚胎性别鉴定的几个关键环节进行了综述,并对其前景进行了展望。Sex identification in embryos is to achieve desired offspring sex ratio and obtain economic profits. There are several methods for sexing embryos, such as karyotype analysis, H-Y antigen detection, X-linked enzymatic determination and polymerase chain reaction (PCR) amplification. PCR-based embryo sexing are the ideal method in the present because it is relatively simple, rapid, and accurate. Sex determining gene, the key steps of sex identification in embryos with polymerase chain reaction and the perspectives were introduced in this article.

关 键 词：胚胎性别鉴定 PCR法 研究进展 PCR扩增法 性别决定基因 经济效益 性别比例 鉴定方法 关键环节 检测法 连接酶 分析法 后代 家畜 抗原 

分 类 号：S813.2[农业科学—畜牧学]