烟草与曼陀罗属间杂交育种研究  

Research on distant hybridization between tobacco and stramonium

作　　者：魏治中[1] 闫新甫[2] 邓志峰[1] 杨俊仙[1] 汪泳涛 

机构地区：[1]山西农业大学,山西太谷030801 [2]国家烟草专卖局,北京100053 [3]山西省灵丘县烟叶服务中心,山西灵丘034400

出　　处：《中国烟草科学》2005年第1期1-5,共5页Chinese Tobacco Science

基　　金：国家烟草专卖局重点项目(国烟科[1999]432号)

摘　　要：利用烟属的普通烟草品种龙烟2号与曼陀罗属的曼陀罗进行属间远缘杂交育种,培育含阿托品及莨菪烷生物碱的新型烟草,满足患哮喘、咳嗽等病症,但又难以戒烟的消费者的需要。采取无性与有性杂交相结合的方法克服杂交不孕。经酶谱及R A PD分子检测,稳定的杂种后代含有双亲的遗传物质;医药成分与单料烟烟气检测结果表明,稳定的杂种后代,都含阿托品、东莨菪碱;镜检结果表明,F1-F18代细胞染色体呈杂合状态;卷烟工业验证其具有较高的利用价值。By means of distant hybridization between Longyan II  a variety of Nicotiana tabacum and stramonium new-type tobacco which contained atropine and scopolamine was expected to be incubated to meet the needs of consumers who were suffering asthma and cough but couldn't stop smoking. The hybridization method of asexuality combined with sexuality was used to overcome the infertility after hybridization. The isoenzyme identification and RAPD analysis showed that the filial generation contained genetic materials from both parents. The detection of medical ingredients and smoke of mono-tobacco cigarette indicated that the filial generation contained atropine and scopolamine. The result of the microscope detection showed that F1-F18 cell chromosome were heterozygosis. It was proved by the cigarette factory after many experiments that the new-type tobacco had comparably high utility value.

关 键 词：曼陀罗 育种研究 属间杂交 远缘杂交育种 杂种后代 细胞染色体 烟草品种 有性杂交 分子检测 RAPD 遗传物质 检测结果 医药成分 东莨菪碱 利用价值 卷烟工业 阿托品 生物碱 消费者 哮喘 病症 咳嗽 不孕 镜检 

分 类 号：S572.035.1[农业科学—烟草工业]