愤怒心理应激大鼠模型的建立及对行为、内分泌免疫的影响  

Establishment of animal model with psychological stress of anger and the effects on behaviour and endocrine-immunity

作　　者：张红梅[1] 刘晓伟[1] 曲宏达[1] 

机构地区：[1]南方医科大学中医系临床基础教研室,广州510515

出　　处：《中华精神科杂志》2005年第2期117-120,共4页Chinese Journal of Psychiatry

基　　金：国家自然科学基金资助项目 ( 30271567 );广东省中医药管理局基金资助项目(02102074)

摘　　要：目的制作愤怒心理应激的动物模型,建立其行为评估体系,观察愤怒应激对大鼠内分泌和免疫系统的影响.方法采用Megan方法制造模型,将32只雄性Wistar大鼠分为正常对照组、愤怒模型组、孤独对照组3个实验组和1个入侵组,每组8只,记录并分析各实验组大鼠的行为变化,检测血液促肾上腺皮质激素(ACTH)、皮质醇、白细胞介素2(IL-2)、白细胞介素8(IL-8)水平,有效评价模型质量.结果 (1)愤怒模型组行为评定总分为(65.88±6.20)分;各行为指标与总分呈中至高度正相关(r=0.363～0.915).(2)旷场实验中的水平得分、垂直得分及总分,愤怒模型组均高于正常对照组和孤独对照组,孤独对照组也高于正常对照组(均P<0.01).行为学观察,与正常对照组比较,愤怒模型组中央格停留时间长,修饰次数和修饰时间少,粪便粒数多;孤独对照组中央格停留时间长,修饰次数(P<0.05)和修饰时间少(均P<0.01).与愤怒模型组比较,孤独对照组中央格停留时间长,修饰次数和修饰时间多,粪便粒数少(均P<0.01).(3)与正常对照组比较,愤怒模型组血浆ACTH和皮质醇高,IL-2低;孤独对照组ACTH高(P<0.01和P<0.05).与愤怒模型组比较,孤独对照组IL-2高(P<0.01).结论该模型是理想的愤怒心理应激模型,模型评估方法可行性较好;愤怒心理应激能够激活丘脑-垂体-肾上腺皮质轴,抑制大鼠的免疫系统功能.Objective To establish the animal model of anger emotion similar to human and set up the system of behavioural evaluation, and observe the influence of anger stress on endocrine-immunity and behaviour.Methods Animal model of anger stress was established according to Megan′s method. The behaviour were recorded and analysed so as to evaluate the model.Results (1) The average score of model group was 65.88±6.20,some items were moderately and highly correlated to total score (r=0.363-0.915). (2) Each index of open field test in model group changed significantly compared with normal control group (P<0.05). (3) Anger stress could significantly reduce the level of adrenocorticotropic hormone (ACTH) and cortisol in plasma (P<0.05), so was IL-2 (P<0.01).Conclusion The established model is an ideal animal model of anger stress, the method of evaluation had better feasibility. Anger stress could activate of HPA pathway and alleviate the function of immune system.

关 键 词：心理应激 内分泌 大鼠模型 白细胞介素8(IL-8) 垂体-肾上腺皮质轴 正常对照组 Wistar大鼠 促肾上腺皮质激素 血浆ACTH 免疫系统功能 IL-2 行为学观察 动物模型 评估体系 行为变化 评价模型 行为评定 应激模型 愤怒心理 

分 类 号：R395[哲学宗教—心理学]