小麦苗期水分利用效率及其相关性状的QTL分析  

QTL Mapping for Water Use Efficiency and Related Traits in Wheat Seedling

作　　者：周晓果[1] 景蕊莲[1] 昌小平[1] 张正斌[2] 

机构地区：[1]农业部作物种质资源与生物技术重点实验室/中国农业科学院作物品种资源研究所,北京100081 [2]中国科学院遗传与发育生物学研究所农业资源研究中心,石家庄050021

出　　处：《植物遗传资源学报》2005年第1期20-25,共6页Journal of Plant Genetic Resources

基　　金：国家863计划(2002AA224011)国家973计划(2003CB114301)资助项目

摘　　要：以小麦DH群体(旱选10号×鲁麦14)为研究材料,采用复合区间作图法,对小麦幼苗在水分胁迫及非胁迫条件下的水分利用效率(WUE)及其相关性状的QTL进行定位,并对比分析QTL的加性效应。两种水分条件下共检测到14个具显著加性效应的QTL,分布在2A、3A、4A、5A、6A、7A、1B、3B、3D染色体上,可解释表型变异的范围在6.36%-19.73%。其中,非胁迫(对照)条件下检测到10个QTL,包括2个单株WUE的QTL,5个地上部WUE的QTL,1个根系WUE的QTL及2个总耗水量的QTL;水分胁迫条件下上述性状各检测到1个QTL。对于同一性状没有检测到在两种水分条件下均位于同一标记区间的QTL,表明不同水分环境条件下同一性状的QTL表达模式是不同的。论文也讨论了可能用于标记辅助选择的QTL及其分子标记。A doubled haploid (DH) population of 150 lines, derived from a cross between two Chinese com mon wheat varieties Hanxuan10 and Lumail4 was used in this study to map quantitative trait loci ( QTL) for water use efficiency (WUE) and related traits at seedling stage under water stress and control conditions, respectively. Mixed-model-based composite interval mapping analysis revealed that a total of 14 QTL of additive effect for the target traits were mapped on chromosomes 2A, 3A, 4A, 5A, 6A, 7A, 1B, 3B and 3D. The contribution range of these QTL to phenotypic variation was from 6.36% to 19.73% . Of these QTL, 10 were de tected under control condition: two for WUE, five for up-ground water use efficiency (UGWUE), one for down-ground water use efficiency (DGWUE) , and two for water consumed (WC); under water stress condition, one QTL was detected for each trait, respectively. There was no QTL regulating the same given trait to be mapped on the same marker interval under both conditions, implying that there were different QTL expression patterns under different water conditions. Some QTL may be employed to perform marker assisted selection in breeding.

关 键 词：相关性状 QTL分析 水分利用效率(WUE) 复合区间作图法 苗期 水分胁迫条件 标记辅助选择 加性效应 水分条件 DH群体 小麦幼苗 对比分析 表型变异 表达模式 环境条件 分子标记 检测 染色体 地上部 耗水量 单株 根系 

分 类 号：S512.1[农业科学—作物学]