离子束诱变产生的CGTase高产菌株发酵规律研究  

Studies on Ferment Rule of the Cyclodextrin Glucanotransferase High-performance Strain Mutated by Ion Beam

作　　者：段宇珩[1] 王雁萍[1] 秦广雍[1] 霍裕平[1] 

机构地区：[1]郑州大学离子束生物工程省重点实验室,河南郑州450052

出　　处：《河南农业科学》2005年第5期15-17,共3页Journal of Henan Agricultural Sciences

基　　金：河南省科技攻关项目 ( 0 0 116 0 2 0 1)

摘　　要：对离子束诱变产生的环糊精葡萄糖基转移酶(CGTase)高产菌株在10L全自动发酵罐中的发酵规律进行研究,结果表明:发酵过程中菌体生长和环糊精葡萄糖基转移酶的产生与发酵过程中pH和溶氧的变化直接相关;菌种传3代,发酵2 4h以内,产酶活力均在6 0 0 0IUThe study was aimed at the ferment rule of the cyclodextrin glucanotransferase high performance strain which was got by nitrogen ion beam injection in 10 L auto fermentor.The result indicated that in the ferment process bacilli growth and cyclodextrin glucanotransferase productivity directly correlated with the change of pH and dissolved oxygen.The enzyme activity of strain 3th generations (F 3)fermented during 24 h were all above 6000IU/ml .

关 键 词：离子束 诱变 环糊精葡萄糖基转移酶高产菌 溶氧 

分 类 号：Q555[生物学—生物化学]