清开灵和生脉注射液联合用药对油酸型急性肺损伤家兔的治疗作用  

Therapeutic effect of Qingkailing and Shengmai injection alone or combined on the acute lung injury induced by oleic acid in rabbits

作　　者：张春颖[1] 杜贵友[1] 梁日欣[1] 王秀荣[1] 崔海峰[1] 赵雍[1] 高伟[1] 吴子伦[1] 贺蓉[1] 高双荣[1] 回连强[1] 朱晓新[1] 

机构地区：[1]中国中医研究院中药研究所,北京100700

出　　处：《中国中药杂志》2005年第9期686-690,共5页China Journal of Chinese Materia Medica

基　　金："十五"攻关项目(2001BA701A66)

摘　　要：目的:观察静脉注射清开灵、生脉注射液(生脉)及其联合用药对油酸型急性肺损伤(ALI)家兔的影响。方法:家兔随机分为11组:对照组,油酸组,治疗组分别包括清开灵和生脉以及清开灵+生脉小、中、大剂量组。iv油酸(0.05mL·kg-1)复制家兔ALI模型后,立即iv上述药液,观察给予油酸前及以后30,60,120min时的呼吸频率和幅度,于各时间点测定动脉血气含量;实验后处死家兔取肺组织,测定肺组织LDH,CAT,MDA的含量及观察病理变化。结果:与油酸组比较,清开灵、生脉及清开灵+生脉的小、大剂量组的呼吸幅度均显著升高;清开灵+生脉小剂量组PaO2显著升高(P<0.05),清开灵、生脉及清开灵+生脉小剂量组的PaCO2均显著降低(P<0.05);清开灵、生脉各剂量组的MDA均显著降低,清开灵+生脉小剂量组的MDA显著降低、CAT显著增加;清开灵、生脉及清开灵+生脉小剂量组可以减轻油酸引起的病理改变。结论:清开灵+生脉小剂量对油酸型肺损伤家兔的疗效优于同剂量下的单独用药。Objective: To investigate the effect of Qingikailing and Shengmai injection alone or combined on the acute lung injury(ALI) induced by oleic acid in rabbits. Method: The rabbits were randomly divided into 11 groups: oleic acid group; control group;treatment groups including low, middle and high dosage groups of Qingkailing and Shengmai injection alone and combined, respectively. ALI model was established by iv oleic acid (0.05 mL·kg^(-1)) in these groups, and then iv above drugs respectively,while in control group, the same volume of normal saline was given. The respiratory amplitude and rate were observed, and blood samples were taken from cervical artery for blood-gas analysis before and at 30, 60, 120 min after oleic acid or normal saline administration. At the end of experiment, the concentration of LDH,CAT and MDA in the lung tissue were measured and pathologic changes of lung tissue were observed microscopically. Result: Compared with oleic acid group,the respiratory amplitude markedly enhanced (P<0.05) in the low and high dose groups of Qingkailing and Shengmai injection. PaO (2) increased significantly(P<0.05)in the low dose group of combined Qingkailing and Shengmai injection, PaCO (2 )decreased markedly(P<0.05)in the low dose groups of Qingkailing and Shengmai injection alone and combined. The level of MDA significantly decreased(P<0.05)in the each group of Qingkailing and Shengmai injection alone, the level of MDA significantly decreased(P<0.05)and CAT increased(P<0.05)in the low dose group of combined Qingkailing with Shengmai injection. The low dose group of combined Qingkailing and Shengmai injection can alleviate the pathological changes induced by oleic acid. Conclusion: The curative effect of the low dose group of combined Qingkailing with Shengmai injection for the ALI induced by oleic acid was better than Qingkailing and Shengmai injection alone at the same dosage.

关 键 词：油酸型急性肺损伤 生脉注射液 清开灵 联合用 家兔 治疗作用 药对 油酸型肺损伤 小剂量 PaCO2 MDA 静脉注射 呼吸频率 动脉血气 病理变化 呼吸幅度 PaO2 病理改变 单独用药 大剂量 肺组织 CAT 对照组 ALI LDH 含量 测定 

分 类 号：R285.5[医药卫生—中药学]