秦川牛和中国荷斯坦奶牛FSHβ基因SSCP多态性分析  

Study on the FSHβ Gene's 5'flank Region of the Qinchuan Ctattle and China Holstein by PCR-SSCP

作　　者：梁宏伟[1] 昝林森[1] 孟彦[1] 方平[1] 田万强[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100

出　　处：《中国农学通报》2005年第5期81-83,共3页Chinese Agricultural Science Bulletin

基　　金：国家农业科技成果转化项目(02EFN216100595);国家农业科技跨越计划(2003-20);国家奶类重大专项(2002BA518A17)资助。

摘　　要：利用SSCP分子标记对秦川牛和中国荷斯坦奶牛的FSHβ基因5’侧翼区进行了多态性分析。结果表明:在秦川牛中检测的FSHβ基因5’侧翼区有AA型、BB型和AB型三种基因型,在中国荷斯坦奶牛中只有AA型。秦川牛中AA基因型的频率为0.395,BB基因型的频率为0.026,AB基因型的频率为0.579,中国荷斯坦奶牛的AA型的频率为1。秦川牛中A的基因频率为0.6842,B的基因频率为0.3158,中国荷斯坦奶牛A的基因频率为1.0。秦川牛群体中多态信息含量为0.3389,多态性高,遗传变异大。The polymorphism of FSHβ was detected in Qinchuan cattle and China Holstein by polymerase chain reaction and 12% PAGE electrophoresis followed by silver staining. The result showed: Three genotype AA, BB and AB were found with the genotype frequency of 0.395,0.026,0.579 in the 5'flank region of FSHβ gene of Qinchuan cattle, but only one genotype with AA in China Holstein. The gene frequency of A and B were 0.6842 and 0.3158 in Qinchuan cattle, but the gene frequence of A was 1.0 in China Holstein. The PIC was 0.3389 in Qinchuan cattle and which means two loci have high polymorphism and large variation.

关 键 词：中国荷斯坦奶牛 FSHΒ基因 多态性分析 SSCP 秦川牛 基因频率 多态信息含量 基因型 AA型 分子标记 遗传变异 AB型 BB型 

分 类 号：S823.912[农业科学—畜牧学] S828.2[农业科学—畜牧兽医]