萃取法提取甘肃棘豆中的苦马豆素研究初报  

Preliminary Reports on Extraction Swainsonine from Oxytropis Kansuensis

作　　者：李勤凡[1] 王建华[1] 刘志斌[1] 蒲鹏[2] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨陵712100 [2]西北农林科技大学生命学院,陕西杨陵712100

出　　处：《中国农学通报》2005年第5期143-145,共3页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金项目"西藏冰川棘豆毒性成分及脱毒机理研究"(30070577);西北农林科技大学科研专项基金"冰川棘豆生物碱系统分析及毒素的微生物降解"(080807)。

摘　　要：将1.5kg甘肃棘豆干粉经水提取,提取液浓缩后用正丁醇萃取,萃取液加入稀硫酸调节pH7.0,回收溶剂,制成浸膏;将浸膏与二氧化硅粉末混匀后用氯仿萃取该浸膏中的小极性杂质。用氨性氯仿萃取,收集氨性氯仿萃取液,挥干后升华得到7.5mg针状结晶,与苦马豆素标准样品对照进行TLC检测,其Rf值及斑点颜色与苦马豆素标准样品一致;通过熔点和IR、MS、1HNMR、13CNMR等鉴定其化学结构,为苦马豆素,计算甘肃棘豆苦马豆素的产率为5mg/kg。1.5kg the Oxytropis Kansuensis powder was extracted by hot water and the liquid was extracted with butanol. Then its adjusted pH to 7.0 and reclaimed butanol. The residue was mixed with silicon dioxide that was extracted with chloroform to remove minimum polarity impurity. In the end, the residue was extracted by ammoniac chloroform, collected ammoniac chloroform phase, vaporized the solvent, sublimated the residue and obtained 7.5mg white needles crystal. The crystal was detected by TLC, which appeared the same dot shape, color and Rf as the standard swainsonine. The chemical structures were identified as swainsonine by means of MP and IR, MS, 1HNMR, 13CNMR..

关 键 词：苦马豆素 甘肃棘豆 研究初报 萃取法 ^13CNMR 二氧化硅粉末 ^1HNMR 标准样品 回收溶剂 针状结晶 化学结构 萃取液 水提取 正丁醇 提取液 稀硫酸 浸膏 氯仿 TLC Rf值 

分 类 号：S856.9[农业科学—临床兽医学] TQ131.13[农业科学—兽医学]