稻纹枯菌酯酶同工酶、可溶性蛋白及致病力的研究初报  

Preliminary study on esterase isozymes,soluble proteins and pathogenicity of different isolates of Rice sheath blight pathogen

作　　者：胡春锦[1] 李杨瑞[2] 黄思良[2] 

机构地区：[1]广西大学农学院,南宁530005 [2]广西作物遗传改良生物技术重点开放实验室,南宁530007

出　　处：《植物病理学报》2005年第1期90-92,共3页Acta Phytopathologica Sinica

摘　　要：对稻纹枯菌25个菌株分别进行了致病力测定、酯酶同工酶和可溶性蛋白分析.结果表明:不同菌株存在明显的致病力分化,而酯酶同工酶图谱却有较强的一致性,它们的主酶带基本相同,但在副酶带上反映出一定的同工酶表型异质性,表现为带的数目及着色深浅的不同;可溶性蛋白图谱比酯酶同工酶图谱表现出更多的多样性,大多数致病力较强的菌株都有l～2条特征谱带,而且它们的谱带数目比弱毒力菌株的多出3～5条.Twenty-five isolates of Rice sheath blight pathogen ( Rhizoctonia solani AG-1-IA) from several different geographic locations were analysed for pathogenicity and electrophoretic profiles of soluble proteins and esterase isozymes.These isolates showed different pathogenicities. The patterns of esterase were similar among these isolates except somewhat difference in stain degree and band's number. However,the patterns of soluble proteins showed remarkable difference. The isolates with relatively strong pathogenicity showed some specific bands and had 3 to 5 more protein bands than the weaker isolates.

关 键 词：酯酶同工酶 可溶性蛋白 纹枯菌 研究初报 稻 致病力分化 蛋白分析 特征谱带 毒力菌株 一致性 异质性 多样性 图谱 酶带 

分 类 号：S646.19[农业科学—蔬菜学] S572[农业科学—园艺学]