杭白菊HPLC指纹图谱的评价  

Fingerprint analysis for the quality assessment of Flos Chrysanthemi

作　　者：蒋惠娣[1] 胡碧波[1] 曾苏[1] 

机构地区：[1]浙江大学药学院药物分析与代谢研究室,浙江杭州310031

出　　处：《中国药学杂志》2005年第8期578-581,共4页Chinese Pharmaceutical Journal

基　　金：浙江省教育厅资助(20030464)浙江省科技厅重点资助(G20020578)

摘　　要：目的建立杭白菊药材的高效液相指纹图谱(HPLC-FP),为杭白菊的鉴别及品质控制提供依据。方法采用RP-HPLC 对不同种植基地、不同时间采摘、不同等级及不同贮存时间的杭白菊与国内其他品种菊花的主要有效成分黄酮类成分进行指纹图谱研究。结果HPLC指纹图谱中杭白菊主要黄酮类成分均得到很好分离,根据聚类分析和相似度分析处理结果,23个不同种植基地、不同采摘时间及不同贮存时间的杭小白菊被正确地区分出来,相似度均在0.95-1.00之间,而其他菊花的相似度均小于或等于0.90。结论HPLC-FP可以作为杭白菊质量评价和药材鉴别的依据。OBJECTIVE: To develop a fingerprint of Flos Chrysanthemi. METHODS: RP-HPLC was used to analyze the flavonoids, the main composition of Flos Chrysanthemi. Agilent Zorbax SB C18 column (250 mm × 4.6 mm, 5 μm) was used as analysis column. The mobile phase was acetonitrile/pH 2.0 phosphate buffer solution with gradient elution. Flos Chrysanthemi of different sources, grades, processes and store time were analyzed by the described methods. RESULTS: By cluster analysis and similarity calculation, twenty-three batches of Fols Chrysanthemi were differentiated, and their similarity were 0.95 ∼ 1.00. The similarity of the other thirteen species were less than 0.90. CONCLUSION: HPLC fingerprint of Flos Chrysanthemi will be an identification method for Flos Chrysanthemi.

关 键 词：杭白菊 指纹图谱 高效液相色谱法 

分 类 号：R2825[医药卫生—中药学] R284[医药卫生—中医学]