鼠抗GM-CSF单克隆抗体的研究  

Study on Murine McAbs to GM-CSF

作　　者：尹红章[1] 饶春明[1] 李秀华[1] 张燕军[1] 张宁[1] 李德富[1] 孟淑芳[1] 

机构地区：[1]中国药品生物制品检定所,北京100050

出　　处：《中国生物制品学杂志》1994年第2期61-63,共3页Chinese Journal of Biologicals

摘　　要：应用基因工程GM－CSF抗原，通过杂交瘤技术获得3株稳定分泌抗GM－CSF的特异性单抗细胞株，分别命名为LD2、4和6。经分析，3种单抗识别抗原分子上的两个不完全相关的位区，为GM－CSF的亲和层析纯化及抗原的免疫学测定提供了必要的条件。3 hybridoma cell strains which could stably secrete McAbs to GM-CSF were obtained by hybridoma technique with recombinant antigens to GM-CSF, and coded LD2, 4 and 6 respectively. The study indicated that the McAbs secreted by the 3 strains could recognize 2 incomplete correlative antigenic determinants. It provided necessary conditions for purification of GM-CSF by affinity chromatography and immunologic ditermination of antigens.

关 键 词：GM-CSF 单克隆抗体 制备 

分 类 号：R392－33[医药卫生—免疫学]