表达狂犬病毒糖蛋白的重组痘苗病毒对啮齿动物接种的反应  

Reaction of Recombinant Vaccinia Virus Expressed Rabies Virus Glucoprotein to Rodents

作　　者：陈迺民 朱家鸿[1] 李萍萍[1] 

机构地区：[1]卫生部武汉生物制品研究所,武昌430060

出　　处：《中国生物制品学杂志》1994年第3期106-108,共3页Chinese Journal of Biologicals

摘　　要：狂犬病毒糖蛋白重组痘菌病毒（V－RG）与非重组痘菌病毒比较，V－RG对小白鼠爆发性致死量下降10000LD50；对家兔眼粘膜及皮肤感染后，3～5天内呈现轻微的红、肿，3周内全部消退。所有局部损害仅限于表皮，未见其它不良反应。V－RG经口免疫的动物7天即产生抗体，于第14天能抵抗致死量狂犬病毒脑内或肌肉内攻击。The toxicity index of rabies virus glucoprotein expressed by recombimant vaccinia virus virus (V-RG) was determined by inoculating mice intracranially, and showed a descent of 10 000LD50 compared with that of vaccina. After rabbits were inoculated with VRG transmucosally and percutaneously, mild redness and swelling were found within 3~5 days and subsided within 3 weeks. All the lessions were located in epiderm, and no other odverse reactions were found. Antibodies could be detected in mice and guinea pigs 7 days after immunization with V-RG perorally, and the animals could resist intracranial and intramuscular challenge with lethal dose of rabies virus 14 days after immunization.

关 键 词：重组痘苗病毒 啮齿目 狂犬病 鉴定 

分 类 号：R392－33[医药卫生—免疫学]