聚乳酸多孔支架制备及细胞实验  

Fabrication and Cell Experiment of Porous PLA Scaffold

作　　者：郭超[1] 张娟[2] 林萍华[1] 董寅生[1] 尹立红[2] 浦跃朴[2] 

机构地区：[1]东南大学材料科学与工程系,南京210096 [2]东南大学医学院,南京210009

出　　处：《中国生物医学工程学报》2005年第1期31-34,25,共5页Chinese Journal of Biomedical Engineering

基　　金：江苏省高新技术研究项目 (BG2 0 0 10 3 3 )。

摘　　要：以冰粒子作为致孔剂 ,采用冷冻干燥 粒子滤出复合法制备了块状聚乳酸多孔支架。将聚乳酸溶于氯仿溶液后加入冰粒子 ,在液氮中冷冻后冷冻干燥获得多孔支架。对支架孔隙结构分析表明 ,该工艺制备的多孔支架无致孔剂残留 ,其孔隙大小由加入的冰粒子大小决定。细胞实验表明该多孔支架具有较好的生物相容性并且无细胞毒性。Using ice particulates as porogen material, massive porous PLA scaffold was fabricated by a method of combining freeze-drying and porogen-leaching. PLA solutions in chloroform were mixed with ice particles, and then freezing in liquid nitrogen, finally, porous scaffold was prepared by freeze-drying. The research of the scaffold's pore structure showed that no porogen remains in the scaffold; the ice particulates size determined the pore size of the scaffold. The result of cell experiment showed that the porous scaffold has good biocompatibility and no cytotoxicity.

关 键 词：组织工程 粒子滤出 冷冻干燥 多孔支架 

分 类 号：R318.08[医药卫生—生物医学工程]