麻黄汤及拆方抗炎作用的研究  

Experimental Study on Anti-inflammatory Effects of Mahuang Decoction and Its Compositions

作　　者：刘永刚[1,2] 罗佳波[1] 贺丰[1] 

机构地区：[1]南方医科大学中药制剂重点实验室 [2]武警广东总队医院药剂科,广州510507

出　　处：《中药材》2005年第5期413-415,共3页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金重点项目(300301500)

摘　　要：目的:通过对麻黄汤各拆方配伍组抗炎作用来探讨其配伍规律。方法:给大鼠腹腔注射糖原诱导中性粒细胞聚集,分离中性粒细胞,以arachidonicacid(AA),calciumionophore(A23187)刺激离体中性粒细胞释放白三烯(LTB4),再用HPLC法测定中性粒细胞中白三烯的含量,并以二甲苯致小鼠耳肿胀研究麻黄汤及拆方的抗炎作用。结果:麻黄汤及拆方不同程度抑制二甲苯致小鼠耳肿胀和中性粒细胞释放白三烯。结论:麻黄汤对二甲苯致小鼠耳肿胀和中性粒细胞释放白三烯具有抑制作用,拆方分析显示麻黄汤全方效果最佳,并初步验证了麻黄汤组方的科学性和合理性。Objective: To explore the regularity of recipe composition by observing anti-inflammatory effects of Mahuang Decoction (MHD) and its compositions. Methods: Neutrophil aggregation was induced by intraperitoneal injection of glycogen in rats. Intraperitoneal lavage fluid(PLF) was collected and neutrophils were removed. Neutrophils were stimulated by calciumionophore A23187 in vitro to produce leukotriene B4. The concentration of leukotriene B4 was measured by reversed-phase HPLC, anti-inflammatory effect was observed by the auricular edema with dimethylbenze. Result: MHD and its compositions could inhibit the auricular edema with dimethylbenze and leukotriene production from neutrophils in rats. Inhibitory effects of MHD compositions were different. Conclusion: MHD had significantly inhibitory effects on the auricular edema with dimethylbenze and leukotriene production from neutrophils in rats. The original formula(MHD) worked best. These result confirmed the rationality and scientific level of MHD.

关 键 词：抗炎作用 麻黄汤 小鼠耳肿胀 中性粒细胞聚集 细胞释放 HPLC法 白三烯 二甲苯 配伍规律 腹腔注射 不同程度 抑制作用 拆方分析 配伍组 合理性 科学性 

分 类 号：R289.5[医药卫生—方剂学] R285.5[医药卫生—中药学]