正交试验法优选火麻仁油中大麻酚类化合物的提取工艺  

Study on the Extraction Process for Cannabinoids in Hemp Seed Oil by Orthogonal Design

作　　者：张岗[1] 郭江宁[2] 毕开顺[2] 

机构地区：[1]东北大学理学院,辽宁沈阳110004 [2]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《中药材》2005年第5期417-418,共2页Journal of Chinese Medicinal Materials

摘　　要：目的:优选火麻仁油中大麻酚类化合物的提取工艺。方法:采用正交实验的方法,以大麻酚和Δ9 四氢大麻酚的含量为指标,来考察提取时间、提取次数和溶剂量对火麻仁油中大麻酚类化合物的提取效果的影响。结果:提取时间每次15min,提取两次,每次加5mL溶剂为最佳提取工艺。结论:该工艺合理、稳定可行,且方法简便。Objective:To select the optimum extracting procedure for cannabinoids from hemp seed oil.Methods: The optimum extracting procedure was selected with the content of cannabinol and Δ9-tetrehydrocannabinol from hemp seed oil by orthogonal test design.We have examined three factors that may influence the extraction rate:the time of extraction,the times of extraction and the amount of methanol.Results: The optimum extraction condition was adding 5 ml,two times amount of methanol into hemp seed oil for 15 min.Conclusion: The above extraction process gave the most rational,stable,feasible and satisfactory results.The method is convenient.

关 键 词：大麻酚类化合物 火麻仁油 正交试验法 优选 △^9-四氢大麻酚 最佳提取工艺 提取时间 正交实验 提取次数 溶剂量 

分 类 号：R979.1[医药卫生—药品] R917[医药卫生—药学]