广东五种菊头蝠的核型分析  

Karyology of Five Species of the Rhinolophus(Chiroptera:Rhinolophidae)from Guangdong, China

作　　者：吴毅[1] Masashi HARADA 

机构地区：[1]广州大学生物系,广州510405 [2]Laboratory Animal Center,Osaka City University Medical School,Osaka,545-8585,Japan

出　　处：《兽类学报》2005年第2期163-167,共5页Acta Theriologica Sinica

基　　金：国家自然科学基金资助项目 (3 0 3 70 167) ;国家留学基金资助项目 (2 1844 0 46)

摘　　要：本文用蝙蝠的新鲜肺组织和尾椎进行组织培养,然后在光学显微镜下计数30个染色体分散良好的中期分裂相细胞,并进行摄影、剪贴和测量。分析了广东地区5种菊头蝠的核型,即小菊头蝠的核型为2n =6 2 ,FN =6 0 ;角菊头蝠的核型为2n =6 2 ,FN =6 0 ;中菊头蝠的核型为2n =6 2 ,FN =6 0 ;大耳菊头蝠的核型为2n =6 2 ,FN =6 0 ;中华(栗黄)菊头蝠的核型为2n =36 ,FN =6 0。大耳菊头蝠的核型为首次报道。角菊头蝠和中菊头蝠的核型与前人报道的结果基本相同。中华(栗黄)菊头蝠的核型(2n =36 )与张维道报道的鲁氏菊头蝠相同,而与印度和斯里兰卡产R rouxii的核型2n =5 6迥异。Chromosome preparations were made from culture cells of primary lung and tail tissue, then determination of the diploid number (2n) was performed on 30 metaphase cells from each specimen. The karyotypes of five species of Rhinolophus bats from Guangdong,China were examined, namely: Rhinolophus pusillus (2n = 62, FN = 60),R.cornutus (2n = 62, FN = 60), R. affinis (2n = 62, FN = 60),R. macrotis (2n = 62, FN = 60),R.sinicus (2n = 36, FN = 60). The karyotypes of R. macrotis is reported for the first time. It is essentially identical to those of previous reports about the karyotypes of R. cornutus,and R. affinis. The karyotype of R.sinicus (2n = 36) is same as R. rouxii sinicus from Anhui reported by Zhang Weidao, but different from that of R. rouxii(2n = 56) from India and Sri Lanka. Finally Rhinolophus diversity in the East Asia is discussed.

关 键 词：核型分析 中菊头蝠 光学显微镜 组织培养 广东地区 斯里兰卡 东亚地区 肺组织 染色体 多样性 中华 蝙蝠 细胞 

分 类 号：Q942[生物学—植物学] Q959.833