益骨胶囊含药血清对大鼠成骨细胞雌激素受体mRNA及其蛋白表达的影响  

Effect of Yigu Capsule Contained Serum on mRNA and Protein Expression of Estrogen Receptor in Osteoblast Rats

作　　者：张荣华[1] 彭柯萍[1] 朱晓峰[2] 蔡宇[1] 黄丰[1] 

机构地区：[1]暨南大学药学院 [2]暨南大学附属第一医院

出　　处：《中国中西医结合杂志》2005年第4期333-337,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金项目 (No.30 4 72 2 74);国家中医药管理局中医药科学研究专项 (No .0 4 - 0 5JL1 9);广东省自然科学基金重点项目 (No .0 4 1 0 5837)

摘　　要：目的从mRNA及蛋白水平研究益骨胶囊含药血清对成骨细胞雌激素受体(ER)表达的影响,从雌激素调控途径探讨益骨胶囊防治骨质疏松症的机理。方法分离、培养新生大鼠成骨细胞,传代后分为3组,即含药血清组、空白血清对照组、DMEM对照组。采用RT- PCR法检测ERα和ERβ的mRNA相对表达量,采用放射性配基结合分析检测ER的亲和力(以平衡解离常数KD表示)和受体数量(以RT表示)。结果益骨胶囊含药血清组和两对照组比较,ERβmRNA的相对表达量增加,差异有显著性(P <0 .0 5 ) ;ERαmRNA表达差异无显著性(P >0. 0 5 ) ;益骨胶囊含药血清组和两对照组比较KD值差异无显著性(P >0 . 0 5 ) ,但RT有提高,差异有显著性(P <0. 0 1)。结论益骨胶囊含药血清能上调成骨细胞ERβmRNA表达,能增加雌激素受体数量。通过雌激素调控途径防治骨质疏松症可能是益骨胶囊的药理机制之一。ObjectiveTo explore the effect of Yigu capsule contained serum on mRNA and protein expression of osteoblast estrogen receptor (ER), for investigating the mechanism of its preventing and treating osteoporosis by means of regulating estrogen. MethodsOsteoblasts separated from newborn SD rats were cultured, and divided into 3 groups after being passaged, i.e. the drug-serum treated group, the blank serum group and the DMEM medium control group. The relative amount of ERα and ERβ expression were determined with RT-PCR, the affinity (expressed by equilibrium dissociation constant, KD) and number of ER (RT) were analysed by radioligand assay. ResultsThe relative amount of ERβ mRNA expression were increased in the drug-serum group, with significant difference as compared with that in the other two groups (P<0.05), but no significant difference among the three groups in ERα mRNA expression (P>0.05). KD in the drug-serum group showed insignificant difference as compared with that in the other two groups (P>0.05), but RT increased in the drug-serum group and the difference in the three groups was significant (P<0.01). ConclusionDrug-contained serum of Yigu capsule can up-regulate the expression of osteoblast ERβ mRNA and increase the amount of ERs. Regulating estrogen is possibly one of the mechanisms of Yigu capsule in preventing and treating osteoporosis.

关 键 词：益骨胶囊 含药血清 雌激素受体MRNA 成骨细胞 蛋白表达 防治骨质疏松症 放射性配基结合 PCR法检测 平衡解离常数 mRNA表达 调控途径 对照组 显著性 ERβ 蛋白水平 新生大鼠 空白血清 DMEM 分析检测 表达差异 药理机制 表达量 

分 类 号：R285[医药卫生—中药学]