人参糖肽对糖尿病大鼠尾腱胶原交联的改善作用  

Ameliorative effect of ginseng glycopeptide on cross-linking of rat tail tendon collagen

作　　者：李才[1] 李相军[1] 苗春生[1] 张秀云[1] 

机构地区：[1]吉林大学再生医学科学研究所,吉林长春130021

出　　处：《中国中药杂志》2005年第7期544-547,共4页China Journal of Chinese Materia Medica

摘　　要：目的:观察人参糖肽对大鼠尾腱胶原交联的影响。方法:用ELISA分析人参糖肽对体外大鼠尾腱胶原交联的抑制效果;在体内给链脲佐菌素诱发的糖尿病大鼠腹腔注射人参糖肽12周后,检测其尾腱胶原在酸溶液中的溶解性、对限制性胃蛋白酶降解性能、SDS2巯基乙醇的可溶性以及人参糖肽对胶原交联的改善作用。结果:在体外人参糖肽能明显抑制胶原交联,人参糖肽可明显提高尾腱胶原在上述溶液中的溶解性,提示人参糖肽能减轻胶原交联的程度。结论:人参糖肽对胶原交联具有明显的改善效果。Objective: To investigate the ameliorative effect of ginseng glycopeptide on cross-linking of rat tail tendon collagen. Method: ELISA was used to determine the inhibitory effect of ginseng glycopeptide on cross-linking of rat tail tendon collagen in vitro . After ginseng glycopeptide was intraperitoneally administrated to streptozotocin-induced diabetic rats for 12 weeks, the acid solubility, limited pepsin degradation properties and solubility in SDS-2-mercaptoethanol of the rat tail tendon collagen were determined, and the effect of ginseng glycopeptide on the tail tendon collagen cross-linking was evaluated. Result: Ginseng glycopeptide inhibited significantly the cross-linking of rat tail tendon collagen in vitro . The solubility of the tail tendon collagen(in acid, pepsin and SDS-2-mercaptoethanol)was markedly decreased in diabetic rats and ginseng glycopeptide-treated diabetic rats had significantly an increase in the collagen solubility in the above-mentioned solutions, suggesting that ginseng glycopeptide decreased severity of the collagen cross-linking. Conclusion: Ginseng glycopeptide exhibits an significantly ameliorative effect on cross-linking of rat tail tendon collagen.

关 键 词：胶原交联 人参糖肽 糖尿病大鼠 改善作用 2-巯基乙醇 尾腱胶原 ELISA 链脲佐菌素 抑制效果 腹腔注射 降解性能 胃蛋白酶 改善效果 溶解性 12周 酸溶液 限制性 可溶性 体外 肽能 

分 类 号：R285.5[医药卫生—中药学]