逐痹胶囊对大鼠佐剂性关节炎的影响  

The effects of Zhubi capsule on adjuvant arthritis in rats

作　　者：李惠[1] 金亚宏[1] 原桂东[1] 王素芬[1] 

机构地区：[1]中国中医研究院中药研究所,北京100700

出　　处：《中国中药杂志》2005年第7期551-554,共4页China Journal of Chinese Materia Medica

摘　　要：目的:观察逐痹胶囊对大鼠佐剂性关节炎(adjuvantarthritis,AA)的影响及作用机制。方法:用福氏完全佐剂(CFA)诱导大鼠AA模型,MTT法检测脾淋巴细胞增殖能力,免疫组织化学方法检测踝关节滑膜白细胞介素1(IL-1),白细胞介素6(IL-6)含量,并且测量原发及继发性关节肿胀程度。结果:生药52,26,13g·kg-1体重灌胃大鼠,逐痹胶囊显著抑制AA大鼠原发及继发踝关节炎性肿胀;降低脾淋巴细胞增殖能力及踝关节滑膜内IL1,IL-6含量。结论:逐痹胶囊对大鼠AA有一定抑制作用。Objective: To explore the therapeutic effects and mechanisms of Zhubi capsule on adjuvant arthritis(AA) in rats. Method: The model of rat was induced by intradermal injection of CFA. The perimeter of ankle joint was measured at different time points. Con A-induced splencyte proliferation was examined by MTT assay and the expression of IL-1, IL-6 in synovium was determined by immunohistochemical method. Result: After the preventive administration of Zhubi capsule(5.2, 2.6, 1.3 g·kg·d -1 ,×25 d ), it was found that Zhubi capsule significantly inhibited the primary and secondary ankle joint swelling. The increased Con A-induced splencyte proliferation reaction and the activated IL-1,IL-6 expression of AA rat were suppressed by the treatment with Zhubi capsule.

关 键 词：大鼠佐剂性关节炎 胶囊 免疫组织化学方法 细胞增殖能力 白细胞介素 福氏完全佐剂 MTT法检测 IL-6含量 关节滑膜 作用机制 诱导大鼠 关节肿胀 炎性肿胀 IL-1 抑制作用 继发性 踝关节 AA 淋巴 原发 

分 类 号：R285.5[医药卫生—中药学] R978.11[医药卫生—中医学]