毛细管电泳-荧光/非接触电导组合型检测器的研制  

Dual Detector with Fluorescence/Contactless Conductometry for Capillary Electrophoresis

作　　者：杨丙成[1] 谭峰[1] 关亚风[1] 

机构地区：[1]中国科学院大连化学物理研究所,大连116012

出　　处：《分析化学》2005年第5期740-742,共3页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金资助项目(No.20227501)

摘　　要：报道了一种毛细管电泳荧光/非接触电导组合型检测器。该检测器共用非接触电导检测池,实现了双检测器响应同步。优化了非接触电导检测系统中激发电压信号及其频率;荧光检测是用发光二极管作为激发光源,用光纤收集并传输荧光信号至光电倍增管。用无机金属离子和异硫氰酸荧光素评价该体系,结果表明,该检测器达到了任一单类型检测器性能指标。A dual detector with fluorescent and contatless conductometric measurements for capillary electrophoresis was constructed and evaluated. The detector shares a common detection zone and has synchronized responses in both detection modes. Conditions of excitation voltage and its frequency on contactless conductometric response were optimized. In fluorescent detection, a high-brighness light-emitting diode ( LED ) of 470 nm was used as excitation source, and an optical fiber was used to collect and transmit the emitted fluorescence to a photomultiplier tube. Model mixture of six inorganic ions and fluorescein isothiocyanate ( FITC ) was used to evaluate the performance of the dual detector in capillary electrophoresis. The results showed that the performance of the detector was the same as those of any individual detector.

关 键 词：毛细管电泳法 检测器 无机离子 非接触电导检测法 荧光检测法 异硫氰酸 

分 类 号：O658.9[理学—分析化学]