一种简便分离土壤棉花黄萎病菌的选择性培养基  

A New Selective Medium to Simplify Isolate Verticillium dahliae in Cotton from Naturally Infested Soil

作　　者：贾涛[1] 杨家荣[1] 

机构地区：[1]西北农林科技大学植保学院,陕西杨凌712100

出　　处：《棉花学报》2005年第3期151-154,共4页Cotton Science

基　　金：欧盟科技合作项目(ICA4CT2004110001)

摘　　要：介绍了一种用于检测土壤棉花黄萎病菌的选择性培养基,其成分为:鲜棉叶100g,蔗糖5g,琼脂17～20g,40%PCNB0.3g,氯霉素、链霉素和青霉素各0.05g,水1000ml,pH6.4～7.0。该培养基与其它常见分离培养基相比,成分简单、操作简便,检测率显著,抑菌效果好,病菌在其上生长快、菌落典型,是一种较为理想的选择性分离培养基。A new selective medium to simplify isolate Verticillium dahliae in cotton from naturally infested soil was introduced. The medium consisted of following ingredients per liter distilled water: cotton leaves 100 g, sugar 5 g, agar 17~20 g, 40% PCNB 0.3 g, chloramphenicol 0.05 g, streptomycin 0.05 g and penicillin 0.05 g, pH adjusted to 6.4~7.0. The medium was simple and quick. The microsclerotia V.dahliae from naturally infested soil could be effectively isolated while it was rarely infected by other pathogens. The colonies of V.dahliae on the medium were large black-colored, and the mean recovery rate of the number of V. dahliae inoculated in soil could reach 82.88%. In addition, the microsclerotia Verticillium dahliae growth speed on this medium was the fastest. At the same time, the optimum determining duration was 21st days after microsclerotia V.dahliae from naturally infested soil were cultured on this medium. Alteration amount of leaves cotton quality in 1000 ml medium had great effect on isolating. When it was low than 0.05 g·ml^(-1) medium, the medium was rarely infested by other pathogens, but the rate of the number of the microsclerotia V. dahliae from naturally infested soil was lower, because there was insufficient nutrition. When it was high than (0.125) g· ml^(-1) medium, although nutrition was adequate for microsclerotia development and the growth speed of V.dahliae on it was faster, the medium was seriously infested by other pathogens and it was gradually changed no transparence, so the rate of the number of the microsclerotia V. dahliae was lower, too.

关 键 词：选择性培养基 棉花黄萎病菌 土壤 简便 分离培养基 抑菌效果 20g 氯霉素 青霉素 链霉素 检测率 成分 种用 棉叶 

分 类 号：S435.621[农业科学—农业昆虫与害虫防治]