棉花钾吸收动力学的初步研究和应用(英文)  

Preliminary Study of K^+ Uptake Kinetics of Cotton (Gossypium hirsutum L.) and its Application

作　　者：张志勇[1] 王刚卫[1] 田晓莉[1] 李召虎[1] 段留生[1] 翟志席[1] 何钟佩[1] 

机构地区：[1]中国农业大学农学与生物技术学院,北京100094

出　　处：《棉花学报》2005年第3期165-170,共6页Cotton Science

基　　金：国家自然科学基金(30100111)

摘　　要：研究了影响棉花钾吸收动力学参数的因素,初步确定了钾吸收动力学方法在棉花上应用的条件,比较了转基因抗虫棉新棉99B和常规棉中棉所35苗期的钾吸收特性。结果表明,棉花钾吸收动力学参数受到苗龄、耗竭液中起始钾浓度及培养液中钾浓度的显著影响,在苗龄较小(3～4叶苗与4～5叶苗相比)、耗竭液中起始钾浓度较高(0.35与0.2mmol·L1相比)、培养液中钾浓度较高(2.0与0.5mmol·L1相比)的情况下,Km、Cmin均比较高,而Imax在前两种条件下较高、在后一种条件下较低。在5～6叶期,新棉99B的Imax显著降低于中棉所35,而Km、Cmin显著高于后者(培养液中K+为0.5mmol·L1),耗竭液中起始K+为0.2mmol·L1),说明新棉99B吸收有限钾的能力低于中棉所35。It has been a main problem in cotton production that transgenic insect-resistant cotton is prone to premature senescence induced by K^+ deficiency, which makes it necessary to study K^+ uptake characteristics and, on the basis of it, to screen cotton cultivars with high K^+ uptake efficiency. This experiment studied the factors influencing K^+ uptake kinetic parameters of cotton, preliminarily identified the conditions of K^+ uptake kinetic applied to cotton, and compared the K^+ uptake characteristics of transgenic insect-resistant cotton cultivar,DP99B,and conventional cotton cultivar,CCRI 35, at seedling stage. The results showed that K^+ uptake kinetic parameters were significantly affected by seedling stage, initial K^+ concentration in depleting solution and K^+ concentration in culturing solution. Higher K_m and C_(min) resulted from younger seedling (3~4 vs. 4~5 leaf stage), higher initial K^+ concentration in culturing solution (0.35 vs. 0.2 mmol·L^(-1)) and higher K^+ concentration in culturing solution (2.0 vs. 0.5mmol·L^(-1)), but higher I_(max) was produced under the two former conditions, and lower I_(max) came into being under the last situation. Compared with CCRI 35, DP99B had significantly lower I_(max) and higher K_m and C_(min) (0.5 mmol·L^(-1) K^+ in culturing solution and 0.2 mmol·L^(-1) initial K^+ in depleting solution) at 5~6 leaf stage, which indicated that the ability of DP99B to uptake limited K^+ was weaker than that of CCRI 35.

关 键 词：研究和应用 棉花 吸收动力学参数 中棉所35 转基因抗虫棉 Imax 动力学方法 钾浓度 培养液 吸收特性 K^+ 钾吸收 常规棉 起始 耗竭 相比 苗龄 种条 苗期 

分 类 号：S562[农业科学—作物学]