人脑源性神经营养因子基因修饰神经干细胞移植对痴呆大鼠学习记忆的改善  

LEARNING AND MEMORY AMELIORATION OF TRANSPLANTATION OF THE NEURAL STEM CELLS MODIFIED WITH HUMAN BRAIN-DERIVED NEUROTROPHIC FACTOR GENE ON ALZHEIMER DISEASE MODEL RAT

作　　者：赵志英[1] 胡海涛[1] 冯改丰[1] 许杰华[1] 张海英[1] 

机构地区：[1]西安交通大学医学院人体解剖学教研室

出　　处：《中国修复重建外科杂志》2005年第5期331-334,共4页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：陕西省自然科学基金资助项目 (2 0 0 1 SM57)~~

摘　　要：目的　观察神经干细胞( neural stem cell,NSC)和人脑源性神经营养因子( human brain derivedneurotrophic factor,h BDNF)基因修饰NSC移植对阿尔茨海默病( Alzheimer disease,AD)模型鼠的学习记忆改善作用。　方法　SD大鼠4 0只随机分为4组,每组10只。 组为正常对照组;其余3组进行单侧切断大鼠穹窿海马伞( fibria-fornix,FF)制备AD模型, 组为损伤组; 、组于术后10～12 d,侧脑室移植h BDNF基因修饰及未修饰的NSC分别为NSC组和BDNF组。移植后2周进行Morris水迷宫定位航行及空间探索行为学检测。　结果　 组和 组的平均逃避潜伏期为4 1.84±2 1.76 s和2 5 .2 3±17.0 6 s,较 组潜伏期70 .91±2 3.6 7s明显缩短,差异有统计学意义( P<0 .0 1) ;平台象限游泳距离占总游泳距离的百分比: 组和 组分别为36 .9%和4 2 .0 % ,较 组2 6 .0 %明显增高,差异有统计学意义( P<0 .0 1) ; 组大鼠采取边缘式和随机式搜索模式显著多于其它各组,差异有统计学意义( P<0 .0 1)。其中 组大鼠的平均逃避潜伏期和平台象限游泳距离百分比及搜索策略接近 组( P>0 .0 5 ) ,采取直线式和趋向式的搜索策略显著多于其它各组( P<0 .0 1)。　结论　NSC和h BDNF基因修饰NSC移植对AD模型鼠的行为学有不同程度的改善,h BDNF基因修饰NSC移植较?Objective To investigate the memory amelioration of the Alzheimer disease (AD)model rat after being transplanted the single neural stem cells(NSC) and NSC modified with human brain derived neurotrophic factor(hBDNF) gene. Methods Forty SD rats were divided evenly into 4 groups randomly. The AD model rats were made by cutting unilaterally the fibria fornix of male rats. Ten to twelve days after surgery, the genetically modified and unmodified NSC were implanted into the lateral cerebral ventricle of group Ⅲ and group Ⅳ respectively. Two weeks after transplantation, the amelioration of memory impairment of the rats was detected by Morris water maze. Results The average escaping latency of the group Ⅲ and group Ⅳ (41 84±21 76 s,25.23±17 06 s respectively) was shorter than that of the group Ⅱ(70.91±23 67 s) ( P <0.01). The percentage of swimming distance in the platform quadrant in group Ⅲ (36 9%) and in group Ⅳ(42 0%) was higher than that in the group Ⅱ(26 0%) ( P <0.01). More marginal and random strategies were used in group Ⅱ.The percentage of swimming distance in the platform quadrant in group Ⅳ was also greater than that in group Ⅲ( P <0 05). There were no significant differences in the average escaping latency, the percentage of swimming distance in the platform quadrant and the probe strategy between group Ⅳ and group Ⅰ( P >0.05).More lineal and oriented strategies were used in group Ⅳ. Conclusion The behavioral amelioration of AD model rat was obtained by transplanting single NSC and hBDNF gene modified NSC. The effect of the NSC group modified with hBDNF gene is better than that of the group Ⅲ.

关 键 词：人脑源性神经营养因子 基因修饰 学习记忆 神经干细胞移植 痴呆大鼠 Morris水迷宫 HBDNF disease 逃避潜伏期 SC移植 factor 阿尔茨海默病 搜索策略 brain 正常对照组 穹窿海马伞 侧脑室移植 行为学检测 AD模型鼠 统计学 

分 类 号：R651.1[医药卫生—外科学]