X线照射对脊髓神经组织效应的实验研究  

An experimental study of the effect of X-ray radiation on spinal neuron and oligodendrocytes

作　　者：金冶华[1] 白希壮[2] 吕刚[2] 张世斌[1] 王星铎[2] 于频[3] 

机构地区：[1]辽宁省人民医院骨科,沈阳市110015 [2]中国医科大学附属第一临床医院骨科,110001 [3]中国医科大学脑研究所,110001

出　　处：《中国脊柱脊髓杂志》2005年第5期308-310,i004-i005,共5页Chinese Journal of Spine and Spinal Cord

摘　　要：目的:探讨X线照射对脊髓神经元及少突胶质细胞的效应。方法:将出生3d的W istar大鼠分为实验组、对照组,实验组分别给予一次剂量25、35、45和55Gy的X线照射腰段脊髓,观察照射后动物一般状态及运动功能情况。取已照射大鼠脊髓标本进行组织学评价,用图像分析仪行定量分析;用透射电镜观察超微结构的变化。结果:25、35Gy照射组动物未出现神经症状,且生长发育正常;45和55Gy照射组新生鼠的被照射区皮肤毛发稀少,食欲差,身体发育不良,比对照组瘦小,双侧后肢完全瘫痪,并出现尿潴留。对照组与实验组以及各实验组间少突胶质细胞数量均有非常显著性差异(P<0.01)。X线照射区与相邻正常区相比少突胶质细胞明显减少,以脊髓白质区减少最为明显;X线照射剂量与少突胶质细胞数目减少程度有极显著正相关;X线照射剂量超过35Gy可以造成脊髓神经元的损伤。结论:X线照射可抑制大鼠脊髓少突胶质细胞生长,为促进脊髓损伤后神经再生机理的研究提供一定的实验基础。Objective: To study the effect of X-ray radiation on spinal neuron and oligodendrocytes.Method:Wistar rats at the third day after birth were divided into experimental and control groups,with X-ray dosage of 25,35,45 and 55Gy once for each on the lumbar segments of the experimental group.The general condition and motion function after radiation were observed.The specimens of spinal cords were obtained for histological examination,quantitative analysis with image analyzer,as well as qualitativly observed with transmission electron microscope.Result:The rats with dosage of 25Gy and 35Gy did not present neural symptom,and developed normally.In 45 and 55Gy groups hair became less in the radiated areas.They also showed bad appetite,dysplasia,thin and small than the control group,and also with uroschesis.There were singnificant differences on numbers of oligodendrocytes between experimental and control group,as well as among experimental groups.There were significant decreases of oligodendrocytes in radiated areas compared with normal areas in the vicinity,most prominent in the white matter.There was extremely significant positive relativity between dosage of radiation and the amount of decrease of oligodendrocytes.The dosage of X-ray over 35Gy might lead to the impairment of spinal neurons.The dosage of 25Gy and 35Gy could lessen the number of oligodendrocytes without injury to the spinal neuron.Conclusion:The inhibition effect of X-ray to oligodendrocytes provided an experimental basis for the research on mechanisms of neuron and spinal cord re-generation following injury.

关 键 词：运动神经元 少突神经胶质 脊髓 X线 

分 类 号：R683.2[医药卫生—骨科学] R814.2[医药卫生—外科学]