异育银鲫实验性免疫抑制模型的建立  

Preliminary study on establishing the experimental immunosuppression model of allogynogenetic silver crucian carp

作　　者：陈勇[1] 周洪琪[1] 余奇文[2] 张继英[2] 沈佰华[2] 柏峻[2] 

机构地区：[1]上海水产大学生命科学与技术学院,上海200090 [2]上海第二医科大学,上海市免疫学研究所上海200025

出　　处：《水产学报》2005年第2期227-231,共5页Journal of Fisheries of China

基　　金：上海市科委重点攻关项目(013212101)

摘　　要：分别以每kg体重鱼注射0、1、10、20 mg环磷酰胺的剂量、每隔5 d给异育银鲫注射1次,共注射3次,第16天检测各组试验鱼外周血白细胞数量及吞噬活性、不同类型的白细胞数量变化、脾脏和头肾的溶菌酶活性、体外培养外周血B淋巴细胞分泌的IgM、IFN-α含量、外周血NK细胞杀伤功能及头肾、脾脏、外周血B淋巴细胞转化能力。结果表明,环磷酰胺剂量的增加可以使试验鱼外周血白细胞的数量包括各种类型的白细胞的数量、体外培养外周血B淋巴细胞分泌的IgM、IFN-α含量下降,脾脏和头肾的溶菌酶活性、外周血白细胞吞噬活性及头肾、脾脏、外周血B淋巴细胞转化能力降低。提出每kg鱼10mg的环磷酰胺是建立异育银鲫实验性免疫抑制模型的适宜剂量。Different doses (0,1, 10 and 20 mg per kg fish) of cyclophosphamide were respectively injected into abdominal cavity of one of four groups of allogynogenetic silver crucian carp every 5 days for three times. Splenic, head kidney and peripheral blood B lymphocytes proliferation, leucocytes count, including different types of leucocytes count in peripheral blood and phagocytic activity , lysozyme activity of spleen and head kidney, IgM and IFN-α content produced by B cell cultivated in vitro and natural killer cell activity in peripheral blood were observed for each group on the 16th day. The results showed that IgM and IFN-α content produced by B cell cultivated in vitro and leucocytes count, including different types of leucocytes count in peripheral blood obviously decreased and that splenic, head kidney and peripheral blood B lymphocytes proliferation, lysozyme activity of spleen and head kidney, natural killer cell activity in peripheral blood and leucocytes phagocytic activity evidently reduced with increasing of dose of cyclophosphamide.It is thought that 10mg·kg-1 is a suitable dose of cyclophosphamide for establishing the experimental immunosuppression model of allogynogenetic silver crucian carp.

关 键 词：异育银鲫 免疫抑制模型 环磷酰胺 

分 类 号：S942.5[农业科学—水产养殖]