造血干细胞移植后巨细胞病毒感染患者糖蛋白B基因分型的初步研究  

A study of cytomegalovirus(CMV)glycoprotein B genotypes in patients with CMV infection after hematopoietic stem cell transplantation

作　　者：吴小津[1] 吴德沛[1] 孙爱宁[1] 马骁[1] 常惠荣[1] 朱子玲[1] 

机构地区：[1]苏州大学附属第一医院江苏省血液研究所,215006

出　　处：《中华内科杂志》2005年第4期290-292,共3页Chinese Journal of Internal Medicine

基　　金：江苏省医学重点人才基金资助项目(RC2002033);江苏省六大高峰人才基金资助项目

摘　　要：目的初步探讨造血干细胞移植(HSCT)后巨细胞病毒(CMV)感染患者CMV糖蛋白(G)B基因分型及其与致病性的关系。方法研究了2001年3月至2003年12月在我院行HSCT的患者38例,均采用巢式PCR方法检测CMV GBDNA为阳性,对PCR产物用RsaⅠ和HinfⅠ内切酶进行了酶切分型。结果Ⅰ型19/38例(50.0%),Ⅱ型3/38例(7.9%),Ⅲ型14/38例(36.8%),Ⅰ与Ⅲ混合型2例。Ⅰ型感染预后良好,Ⅲ型与致死性间质性肺炎(IP)发生有明显的相关性。Ⅰ、Ⅲ型患者移植物抗宿主病(GVHD)的发生率差异无统计学意义。结论CMVGB蛋白的基因分型与CMV的IP发生有关,与GVHD的发生无明显相关性。Objective To study the different cytomegavirus (CMV) glycoprotein(G) B genotypes in the patients with CMV infection after hematopoietic stem cell transplantation (HSCT) and the correlation between genotype and pathogenesis of CMV disease.Methods Thirty-eight patients received allogenetic from March 2001 to December 2003. CMV GB DNA was detected with nested-PCR in all the patients and the products were digested with RsaⅠand HinfⅠ. Results The distribution of GB types were as follows: GB1, 19/38 (50.0%);GB2, 3/38 (7.9%), GB3, 14/38 (36.8%)。GB1 was associated with favorable clinical outcome, while GB3 mighte contribute to fatal interstitial pneumonia(IP). The incidences of graft versus host disease (GVHD) were not different significantly between the patients with GB1 and those with GB3.Conclusion CMV GB genotypes might play an important role in the pathogenesis of CMV-IP, but not of GVHD.

关 键 词：基因分型 感染患者 糖蛋白B 巨细胞病毒(CMV) 步研究 移植后 造血干细胞移植 巢式PCR方法 移植物抗宿主病 2003年 2001年 HinfⅠ PCR产物 间质性肺炎 初步探讨 HSCT 酶切分型 GVHD 相关性 致病性 DNA 内切酶 Rsa Ⅲ型 混合型 

分 类 号：R55[医药卫生—血液循环系统疾病]