黄芩苷对胰岛细胞瘤细胞系增殖的影响  

Effect of baicalin on the proliferation of insulinoma cell line

作　　者：宋健辉[1] 孙吉平[1] 贾延劼[2] 杨于嘉[1] 

机构地区：[1]中南大学湘雅医院儿科,长沙410008 [2]河南医科大学第一附属医院神经内科,郑州450052

出　　处：《中南大学学报（医学版）》2005年第2期145-148,共4页Journal of Central South University ：Medical Science

基　　金：国家自然科学基金(No. 30200128)

摘　　要：目的:探讨黄芩苷对胰岛细胞瘤细胞的影响及其分子机制。方法:应用光学显微镜、MTT测定、流式细胞仪和Westernblotting等方法,研究0, 100, 200, 400μg/ml黄芩苷处理胰岛细胞瘤细胞24h,或200μg/ml黄芩苷处理胰岛细胞瘤细胞不同时间后,黄芩苷对细胞增殖速度、细胞存活率、细胞周期分布的影响。结果:随黄芩苷浓度增加,细胞增殖受到抑制,细胞的生存率逐渐下降;随着黄芩苷作用时间的延长,细胞的生存率也逐渐下降;流式细胞仪结果显示,随着黄芩苷处理浓度增加,S期细胞明显减少,从38. 2% ( 0μg/ml)下降至9. 4% ( 400μg/ml),G0 /G1期细胞所占百分率增加,从56. 4% (0μg/ml)上升至85. 9% (400μg/ml),细胞出现G1阻滞。同时,Westernblotting结果显示细胞周期蛋白cyclinD1表达显著下调。结论:黄芩苷能抑制胰岛细胞瘤细胞株增殖;黄芩苷诱导所致细胞周期蛋白cyclinD1表达下调在其中起着重要作用。Objective To investigate the effect of baicalin on the proliferation of insulinoma cell line and the molecular mechanism involved. Methods Such methods as light microscope, MTT assay, flow cytometry and Western blotting were applied to investigate the effects of baicalin(0, 100, 200, and 400μg/ml baicalin treated for 24h or 200μg/ml baicalin treated at different time points)on the cell proliferation, cell survival rate, the cell cycle and related molecular mechanisms. Results The number of proliferating cells obviously decreased with the increase of baicalin under the light microscope, and the survival rate of cells decreased as determined by MTT assay. After being treated with baicalin, the number of insulinoma cells in S-phase obviously decreased from 38.2%(0μg/ml) to 9.4%(400μg/ml), and the number of cells in phase G1 increased from 56.4%(0μg/ml)to 85.9%(400μg/ml). In the meantime, the expression of cyclin D1 was obviously declined by Western blotting. Conclusion Baicalin can inhibit the proliferation of insulinoma cells, and the down-regulation of the expression of cyclin D1 might also be involved in these events.

关 键 词：黄芩苷 细胞系增殖 胰岛细胞瘤 blotting Western cyclin 细胞周期蛋白 流式细胞仪 细胞周期分布 瘤细胞株增殖 光学显微镜 MTT测定 细胞存活率 分子机制 不同时间 增殖速度 细胞增殖 作用时间 S期细胞 G1阻滞 表达下调 

分 类 号：R285[医药卫生—中药学]