日本血吸虫新基因的克隆及分析  

Cloning and characterization of new genes of Schistosoma japonicum

作　　者：陈欲晓[1] 唐连飞[2] 章洁[3] 袁仕善[3] 曾宪芳[3] 易新元[3] 

机构地区：[1]中南大学湘雅医学院基础医学院免疫学教研室,长沙410078 [2]湖南出入境检验检疫局,长沙410007 [3]中南大学湘雅医学院基础医学院寄生虫教研室,长沙410078

出　　处：《中南大学学报（医学版）》2005年第2期167-170,共4页Journal of Central South University ：Medical Science

基　　金：世界卫生组织 /热带病研究与训练规划署(980268)

摘　　要：目的:克隆并分析日本血吸虫(Schistosomajaponicum,Sj)新基因,提供有效的疫苗候选分子。方法用Sj雄虫可溶性抗原免疫家兔血清为探针筛选Sj成虫cDNA文库,将所获阳性克隆的插入片断进行PCR扩增并测序,通过互联网NCBIGenBank和蛋白质分析软件进行分析。结果:共筛选得11个阳性克隆,经分析有2个新基因,分别是Sj P8(GenBank注册号AF517843)和Sj肌球蛋白cDNA序列(GenBank注册号AY770506),分别编码含75个氨基酸的跨膜蛋白和含212个氨基酸的蛋白片断。结论:Sj P8和Sj肌球蛋白cDNA序列可能为日本血吸虫疫苗的研究提供有价值的材料。Objective To clone and characterize new genes of Schistosoma japonicum, Sj , and to provide efficient vaccine candidates. Methods Sj adult cDNA library was screened with rabbit sera raised against male worm soluble antigen. The inserted cDNA fragments from the positively selected clones were amplified with PCR and further sequenced, as well as characterized through internet NCBI GenBank software. Results Eleven positive clones were obtained and two were verified by GenBank as new, including a novel gene designated as Sj -P8 (GenBank accession No. AF517843) and a new partial cDNA of Sj myosin (GenBank accession No. AY770506). The two new genes encoded a transmembrane protein of 75 amino acids and a myosin protein fragment of 212 amino acids respectively. Conclusion The newly obtained genes may provide useful information for the research on Sj vaccine.

关 键 词：日本血吸虫 新基因 成虫CDNA文库 cDNA序列 阳性克隆 肌球蛋白 PCR扩增 血吸虫疫苗 探针筛选 抗原免疫 NCBI 分析软件 跨膜蛋白 氨基酸 兔血清 可溶性 互联网 蛋白质 片断 注册 

分 类 号：R383.24[医药卫生—医学寄生虫学] R-05[医药卫生—基础医学]