牛颈静脉经不同方法交联改性后的免疫原性  

Immunogenicity of bovine jugular vein conduits treated with different cross-linking methods

作　　者：王晖[1] 胡建国[1] 吴忠仕[1] 胡铁辉[1] 邓又华[1] 冯耀光[1] 姜志斌[1] 杨彦青[1] 

机构地区：[1]中南大学附属湘雅二医院心胸外科,长沙410011

出　　处：《中南大学学报（医学版）》2005年第2期176-178,共3页Journal of Central South University ：Medical Science

基　　金：湖南省科技计划重大专项基金(04Sk1005)

摘　　要：目的:研究牛颈静脉带瓣管道经不同方法交联后免疫原性的变化。方法:将牛颈静脉随机分为4组(n=10):多聚环氧化合物(PC)交联组、染料介导光氧化(DMP)交联组、戊二醛(GA)交联组及新鲜牛颈静脉对照组。制备牛颈静脉匀浆,与完全福氏佐剂混合制成复合乳剂抗原,免疫新西兰大白兔。采用双向免疫扩散法测定兔血清抗体滴度。取新鲜牛颈静脉免疫的兔血清,采用免疫印迹法(Westernblotting)体外检测交联后的牛颈静脉组织匀浆的抗原组分。结果:免疫印迹显示新鲜牛颈静脉组织有特异性重复出现的阳性条带,经PC,DMP交联处理的牛颈静脉均未出现明显阳性条带,经GA交联处理的牛颈静脉中1例出现阳性条带,但无特异性重复出现的条带,各交联处理组间免疫原性无明显差异。结论: PC,DMP及GA交联处理的牛颈静脉可有效地灭活其免疫原性,适合临床应用要求。Objective To evaluate the immunogenicity of bovine jugular vein conduits (BJVCs) treated with different cross-linking methods. Methods The BJVCs were treated with glutaradehyde (GA), dye-mediated photooxidation (DMP) and polyepoxy compound (PC) ( n =10). The tissue homogenates obtained from BJVCs treated with PC,GA,DMP, and fresh BJVCs, were mixed with the complete Freand adjavant to form the emulsive antigen, which were used to immunize rabbits correspondently. The antibody concentrations to BJVCs in those rabbits′ serum were measured by counter double immuno diffusion. The immunologic responses to the BJVCs in different groups were measured with Western blotting. Results The positive bands appeared when the sera of rabbits were immunized by fresh BJVCs reacted with antigens of fresh BJVCs, but no bands appeared when the sera of rabbits were immunized by fresh BJVCs reacted with those antigens of the BJVCs treated with GA, DMP, and PC in Western blotting. Conclusion The immunogenicity of BJVCs treated with PC, DMP, and GA can be reduced significantly and meet the clinical standard.

关 键 词：免疫原性 交联 牛颈静脉带瓣管道 多聚环氧化合物 改性 脉经 新西兰大白兔 血清抗体滴度 阳性条带 免疫扩散法 免疫印迹法 复合乳剂 福氏佐剂 组织匀浆 体外检测 应用要求 特异性 DMP 出现 光氧化 对照组 新鲜 戊二醛 兔血清 

分 类 号：R392[医药卫生—免疫学]