静脉移植物巨噬细胞浸润途径的实验研究  

Pathway for macrophage invasion into the tunica media and the intima in vein graft

作　　者：伍校琼[1] 蔡维君[1] 罗学港[1] 

机构地区：[1]中南大学湘雅基础医学院人体解剖学与神经生物学系,长沙410078

出　　处：《中南大学学报（医学版）》2005年第2期179-182,共4页Journal of Central South University ：Medical Science

基　　金：湖南省自然科学基金资助项目(02JJY2047)

摘　　要：目的:探讨外膜是否是静脉移植物巨噬细胞浸润的一条重要途径。方法:应用免疫荧光组织化学技术对30个静脉移植物再塞标本中CD68(巨噬细胞的标记物)、CD31(内皮细胞的标记物)的表达进行了检测,用激光共聚焦显微镜拍片,图片用SiliconGraphicsOctane进行处理,并对CD68阳性细胞进行计数。结果:在正常静脉血管,外膜中有少量CD68阳性细胞,中膜和内膜中少见;在病变的血管中,CD68免疫阳性细胞在外膜、中膜和内膜都可观察到,与正常血管相比差异有统计学意义(P<0. 05),且有从外膜向中膜和内膜迁移的趋势;中膜CD68免疫阳性细胞的分布和中膜毛细血管的生成过程密切相关。结论:外膜可能是静脉移植物巨噬细胞浸润的一条重要途径。Objective To test whether the macrophages in vein grafts may invade into the vascular wall from the adventitia apart from the lumen. Methods Expression of CD68 (a marker of macrophage) and CD31 (a marker of endothelial cells) in 30 vein grafts were detected by confocal immunofluroscence.Images were processed with Silicon Graphics Octane. Results In normal veins, there were a few CD68 positive cells in the adventitia, but few CD68 positive cells were found in the media and the intima. In the diseased vein, CD68 positive cells were significantly increased and present in all layers of the vascular wall, the adventitia, media and intima, extending from the adventitia to the media. There was a close relationship between the penetration of CD68 positive cells into the media from the adventitia and the angiogenesis in the media. Conclusion The adventitia may be an important pathway for macrophage invasion.

关 键 词：巨噬细胞浸润 静脉移植物 实验研究 CD68阳性细胞 激光共聚焦显微镜 免疫阳性细胞 组织化学技术 免疫荧光 内皮细胞 静脉血管 正常血管 毛细血管 标记物 外膜 中膜 内膜 统计学 

分 类 号：R654[医药卫生—外科学]