Graves病合并2型糖尿病遗传易感性与HLA-DQB1基因多态性  

Study on the Relationship between HLA-DQB1 Gene Polymorphism and the Genetic Susceptibility to Graves' Disease Combined Type 2 Diabetes

作　　者：吕玲[1] 高玉琪[2] 黄繁嫱[3] 李代红[3] 刘纯[3] 刘平[3] 

机构地区：[1]天津市儿童医院,300074 [2]天津医科大学总医院 [3]天津市第一中心医院

出　　处：《天津医药》2005年第5期271-272,共2页Tianjin Medical Journal

摘　　要：目的:研究Graves病合并2型糖尿病患者HLA-DQB1基因多态性。方法:采用序列特异性引物的聚合酶链式反应(PCR-SSP)方法,检测患者组外周血白细胞基因组DNA的HLA-DQB1位点的基因多态性并与健康人对照(对照组)。结果:HLA-DQB10602的基因频率在Graves病合并2型糖尿病患者组中较健康对照组明显增加,Pc<0.01,RR=9.889,有很强的相关性。结论:HLA-DQB10602可能是Graves病合并2型糖尿病患者的易感基因。Objective: To study HLA-DQB1 gene polymorphism in patients with Graves' disease combined with type 2 diabetes. Methods: HLA-DQB1-DNA typing was performed using the polymerase chain reaction method. The DNA was extracted from peripheral blood leucocytes using carbamidine hydrochloric acid methods. Results: The frequency of DQB10602 was significantly increased in the patients with Graves' disease combined with type 2 diabetes compared with controls (Pc < 0.01,RR = 9.889). Conclusion: The result indicated that HLA-DQB10602 allele was significantly associated with Graves' disease combined with type 2 diabetes.

关 键 词：Graves病 基因多态性 HLA-DQB1 遗传易感性 2型糖尿病患者 聚合酶链式反应 序列特异性引物 基因组DNA 外周血白细胞 健康对照组 基因频率 易感基因 健康人 相关性 

分 类 号：R581.1[医药卫生—内分泌] R541.4[医药卫生—内科学]