三七总皂甙对单侧输尿管梗阻后大鼠肾间质纤维化的防治作用  

Effects of Panax Notoginseng Saponins on the Process of Renal Interstitial Fibrosis After Unilateral Ureteral Obstruction in Rats

作　　者：苏白海[1] 李孜[1] 樊均明[1] 王宓[2] 唐嵘 

机构地区：[1]四川大学华西医院肾脏科,成都610041 [2]北京医科大学第三医院肾内科 [3]北京健康在线网络技术有限公司

出　　处：《四川大学学报（医学版）》2005年第3期368-371,共4页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金 (批准号 3 0 170 43 7);四川省科委基金(批准号 C990 47)资助

摘　　要：目的　研究三七总皂甙对单侧输尿管梗阻后大鼠肾间质纤维化过程的防治作用。方法　随机分为假手术组、手术组和三七总皂甙治疗组,建立单侧输尿管梗阻肾间质纤维化模型,分别于造模后第3、7、14、2 1d处死大鼠,留取梗阻侧肾脏制备病理切片。光镜观察肾间质病理形态学变化;免疫组织化学染色法检测增殖细胞核抗原(PCNA)和α-平滑肌肌动蛋白(α- SMA)表达。结果　手术组呈进行性肾间质纤维化,肾小管上皮细胞及间质α-SMA表达明显增加。三七总皂甙治疗组肾间质纤维化减轻,肾小管上皮细胞PCNA表达明显增加,α- SMA表达明显减少,间质PCNA、α- SMA表达明显减少(P<0 .0 5 )。结论　三七总皂甙对单侧输尿管梗阻后的肾间质纤维化有抑制作用,可阻断肾小管上皮细胞-肌纤维母细胞的转分化。Objective To evaluate the effects of panax notoginseng saponins(PNS) on the process of renal interstitial fibrosis after unilateral ureteral obstruction in rats. Methods In this study, female sprague-Dawley rats were randomly divided into 3 groups: Sham operation group(S group), Operation group(O group), and Operation plus PNS group (P group). The unilateral ureters of the rats were ligated as the operation to create renal interstitium fibrosis. The rats were sacrificed at postoperative days 3,7,14 and 21. After sacrifice, the obstructed kidneys of the rats were sliced to make pathological sections. The sections observed under light microscope to evaluate the results of hematoxylin and eosin (HE) staining, Masson staining or proliferative cell nuclear antigen(PCNA) and α-smooth muscle actin(α-SMA) detected by immunohistochemistry. Results After operation, the operated rats showed progressive renal interstitium fibrosis. The expression of α-smooth muscle actin in renal tubular epithelial cell and interstitium increased significantly. In comparison with the rats of operation group, the PNS-treated rats had less α-smooth muscle actin in interstitium. In addition, PNS could significantly increase the number of cell expressed proliferative cell nuclear antigen in tubules. Conclusion The lesions of tubules-interstitium got worse and the renal interstitium showed progressive fibrosis gradually in rats with unilateral ureteral obstruction. PNS was noted to have ameliorative effects on the fibrotic process of renal interstitium; it can block tubular epithelial-myofibroblast transdifferentiation.

关 键 词：三七总皂甙 增殖细胞核抗原 Α-平滑肌肌动蛋白 肾间质 纤维化 

分 类 号：R285[医药卫生—中药学]