磷酸二酯酶基因在小型猪骨骼肌组织的表达  

Expression of Phosphodiesterase Genes of Skeletal Muscle Tissue in Miniature Pig

作　　者：谷金妮[1] 许剑琴[1] 陈武[2] 于同泉[3] 

机构地区：[1]中国农业大学动物医学院,北京100094 [2]北京农学院动物科学技术系,北京102206 [3]北京市农业应用新技术重点实验室,北京102206

出　　处：《畜牧兽医学报》2005年第5期434-439,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金项目(30270978);北京市科技新星项目(9558100500)

摘　　要：提取中国实验用小型猪骨骼肌组织总RNA,应用反转录聚合酶链反应测定磷酸二酯酶(PDE)同工酶在骨骼肌组织中的表达分布。结果可见PDE1A、1C、2A、3A、3B、4A、4B、4C、4D、5A、7A、7B、8A、8B、9A和11A共16种PDE同工酶mRNA在中国实验用小型猪骨骼肌组织中表达,其中PDE1A、1C、2A、3A、4B、4C、8A和8B共8 种在人和其他动物骨骼肌组织中的表达分布未见报道,PDE1B和10A 3种同工酶未见表达。16种PDE同工酶mRNA在骨骼肌组织中的表达,从电泳条带上可以看到各种PDE表达量存在着差异,但需要进一步做定量分析加以评定。Expression of phosphodiesterase (PDE) isoenzymes was detected in the skeletal muscle tissue in China experimental minature pig in order to seek predominant phosphodiesterase isoenzymes, which is related to development of muscle tissue, and to screen out the selective PDE inhibitors from Chinese herbal medicines, which can improve animal growth and productivity. In this experiment, total RNA was isolated from muscle tissue, the expression of PDE isoenzymes gene was then analyzed by reverse transcript polymerase chain reaction (RT-PCR). 16 PDE isoenzymes mRNA were detected in muscle tissues of China experimental minature pig, i.e. PDE1A, 1C, 2A, 3A, 3B, 4A, 4B, 4C, 4D, 5A, 7A, 7B, 8A, 8B, 9A and 11A except PDE1B and 10A. Among them expression of PDE1A,1C,2A,3A,4B,4C,8A and 8B mRNA have not been reported in the muscle tissue of human and other animals. The different expression extent of the 16 PDE genes were discovered from the electrophoresis bands and need to be elucidated by quantitative detection.

关 键 词：肌组织 小型猪 酯酶基因 反转录聚合酶链反应 组织总RNA mRNA 磷酸二酯酶 同工酶 PDE 动物骨骼 定量分析 骨骼肌 表达量 实验 中国 分布 

分 类 号：S945.26[农业科学—水产养殖] S828[农业科学—水产科学]