体细胞克隆猪的研究与展望  

Strategy and Utilization of Cloned Pig by Nuclear Transfer from Somatic Cells

作　　者：刘珠果[1] 邓继先[1] 

机构地区：[1]军事医学科学院生物工程研究所,北京100071

出　　处：《畜牧兽医学报》2005年第5期506-510,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家高技术发展计划"863"重大专项(2002AA206621)

摘　　要：用体细胞核移植的方法生产的克隆猪诞生至今已近5年,虽然同胚胎细胞核移植相比,体细胞核移植技术难度大,成功率低,但近年来用体细胞核移植技术克隆猪有了更深入的研究,在体细胞核移植基本机理和关键技术上取得了一定的进展。综述了用核移植的方法生产体细胞克隆猪的技术关键和难点及应用情况,并对提高猪体细胞核移植效率的策略进行了分析。It has been almost five years since the first report of live pig produced by somatic cell nuclear transfer (SCNT) technology. Though it is more difficult, lower efficiency, compared to embryonic cell nuclear (ECNT) technology, the cloning pigs with SCNT technology has been further studied. Some progress has taken in the fields of mechanism and approaches. In this paper, we mainly focus on the key technology and its difficulties of SCNT in cloning pigs, including its applications and approach of increasing the efficiency of SCNT.

关 键 词：克隆猪 体细胞核移植技术 展望 技术难度 关键技术 基本机理 应用情况 移植效率 

分 类 号：S814.8[农业科学—畜牧学] Q785[农业科学—畜牧兽医]