白介素-1β诱导和激活许旺细胞的实验研究  

Experimental study on the role of IL-1βin Schwann cells inducing and activeation

作　　者：杨俊[1] 余磊[1] 邱小忠[1] 武雷[1] 陆云涛[1] 刘晓静[1] 廖华[1] 朴英杰 钟世镇[1] 秦建强[1] 

机构地区：[1]第一军医大学临床解剖学研究所,广东省组织构建与检测重点实验室,广州市510515

出　　处：《中华显微外科杂志》2005年第2期142-144,共3页Chinese Journal of Microsurgery

基　　金：国家自然科学基金资助项目(39970833)广东省科技计划专项(2003A3020101)

摘　　要：目的研究白介素-1β对许旺细胞的诱导和激活及其最佳作用浓度.方法取3～4d SD乳鼠坐骨神经,纯化培养许旺细胞,A组加入含10%胎牛血清的DMEM/F 12培养基,B组加入自体神经匀浆激活的巨噬细胞条件培养基;C组加入细胞培养级生物制剂白介素-1β(2.0 ng/ml),分别培养许旺细胞6 d.倒置显微镜观察许旺细胞的形态,抗S-100蛋白免疫组化染色鉴定许旺细胞,MTT法筛选出白介素-1β促许旺细胞分裂增殖作用的最佳浓度,methyl-3H掺入法检测A、B、C组对许旺细胞的促增殖情况,ELISA法检测A、B、C组对许旺细胞NGF分泌量影响.结果 B、C组均比A组更能促进许旺细胞发生分裂增殖并高表达NGF,但以C组的作用最强.结论许旺细胞自身能够表达NGF,而2.0 ng/ml白介素-1β能更好的促进许旺细胞的分裂增殖促进对NGF的高表达.Objective To explore IL-1β induce and activate on Schwann cells and it's the best action concentration. Method To take sciatic nerves in 3-4 days SD rats and purify Schwann cells. A group: DMEM/F12 contained 10% calf blood serum. B group: conditioned medium of macrophages activated by self-neural homogenate. C group: cell culture biological agent IL-1β(2.0ng/ml). Cultured Schwann cells 6 days respectively. Observed morphology of Schwann cells by invert microscope and evaluated Schwann cells in immunocytochemistry staining with anti-S-100. Screened the best concentration of IL-1βwhich promoted proliferation of Schwann cells. To detect A, B and C groups which promoted proliferation of Schwann cells by incorporated radioactive methyl-~3H into culture. Enzyme-linked immunoadsordent assay(ELISA) was detected in A,B and C groups which effected on NGF excretion of Schwann cells. Results B and C groups can be more promoted segmentation and proliferation and higher expression of Schwann cells, but C group is the best. Conclusion Schwann cells can be expressed NGF by itself while 2.0ng/ml IL-1βcan be more promoted segmentation and proliferation and higher expression of Schwann cells.

关 键 词：白介素-1Β 许旺细胞 实验研究 激活 ELISA法检测 条件培养基 NGF 坐骨神经 SD乳鼠 纯化培养 DMEM 胎牛血清 巨噬细胞 自体神经 生物制剂 细胞培养 微镜观察 染色鉴定 免疫组化 最佳浓度 增殖作用 细胞分裂 MTT法 细胞发生 

分 类 号：R651.3[医药卫生—外科学]