SARS病毒刺突蛋白片段在大肠杆菌中的表达和纯化  

Expression of Spike Protein Fragment of SARS Virus in E.coli and Purification of Expressed Product

作　　者：方勇[1] 张秀霞[2] 王宣军[2] 王志武[2] 李洋[1] 盛军[2] 

机构地区：[1]吉林大学生命科学学院,长春130012 [2]长春生物制品研究所,长春130062

出　　处：《中国生物制品学杂志》2005年第3期215-217,共3页Chinese Journal of Biologicals

摘　　要：目的获得SARS病毒表面刺突蛋白片段。方法用PCR方法克隆SARSS2片段基因,并在大肠杆菌中进行表达。表达产物经SDSPAGE及Westernblot鉴定,阴离子交换层析纯化。结果SARS病毒S2片段基因在大肠杆菌中获得了高表达,表达量占总蛋白的50%以上;得到纯度大于90%的SARS蛋白样品。结论获得了能够应用于SARS疫苗研究的SARS蛋白样品。Objective To obtain the spike protein fragment of SARS virus and use for the development of SARS vaccine.Methods The gene encoding S2 fragment of SARS virus was amplified by PCR and expressed in E.coli.The expressed product was identified by SDS-PAGE and Western blot and purified by DEAE-Sepharose FF ion exchange chromatography.Results The gene encoding S2 fragment of SARS virus was highly expressed in E.coli.The expressed product contained more than 50% of total somatic protein and reached a purity of more than 90% after purification.Conclusion The SARS virus protein for developing vaccine was obtained.

关 键 词：大肠杆菌 片段 SDS-PAGE Western 毒刺 SARS病毒 PCR方法 阴离子交换 表达产物 BLOT 层析纯化 S蛋白 高表达 总蛋白 表达量 基因 样品 克隆 

分 类 号：Q78[生物学—分子生物学] TQ610.499[化学工程—精细化工]