CpG-ODN对乙肝核酸疫苗及重组疫苗免疫效果的影响  

Influence of CpG-ODN on Immune Effects of Hepatitis B DNA Vaccine and Recombinant Hepatitis B Vaccine

作　　者：吴晓娟[1] 赵大鹏[1] 冷梅[1] 刘丹[1] 戚凤春[1] 汪春义[1] 王佳凤[1] 岳丹[1] 王华[1] 齐影[1] 付学奇[2] 

机构地区：[1]长春生物制品研究所,长春130062 [2]吉林大学生命科学院,长春130021

出　　处：《中国生物制品学杂志》2005年第3期232-235,共4页Chinese Journal of Biologicals

摘　　要：目的研究CpG寡聚脱氧核苷酸(CpGODN)对乙肝核酸疫苗及重组(CHO细胞)疫苗免疫效果的影响。方法核酸疫苗实验组用CpGODN与乙肝核酸疫苗联合免疫BALBc小鼠,阳性对照组注射核酸疫苗,阴性对照组注射质粒pVAX1;重组疫苗分4个实验组,用CpGODN与不同剂量的重组疫苗配伍后分别免疫BALBc小鼠,阳性对照组注射重组疫苗,阴性对照组注射生理盐水。应用ELISA检测小鼠血清中的抗HBs水平。结果CpG与乙肝核酸疫苗组抗体阳转率比阳性对照组提高3倍,抗体水平提高2倍。CpG与重组疫苗组的阳转率和阳转时间均高于或早于CpG与核酸疫苗实验组。加入CpGODN后,即使重组疫苗用量减少3倍,诱导抗体水平和阳转率均无明显改变。结论CpGODN可提高乙肝核酸疫苗和重组疫苗的免疫效果,并使抗体产生的时间提前。Objective To study the influence of CpG-ODN on the immune effects of hepatitis B(HB) DNA vaccine and recombinant hepatitis B vaccine.Methods Divide 30 BALB/c mice into 3 groups,10 for each,and immunize with CpG-ODN+HB DNA vaccine,HB DNA vaccine (positive control) and plasmid pVAX1(negative control) respectively.Divide another 48 BALB/c mice into 6 groups,8 for each.The mice in 4 test groups were immunized with CpG-ODN combined with recombinant HB vaccine at different dosages respectively,using recombinant HB vaccine as positive control and physiological saline as negative control.The anti-HBs levels in sera of mice were detected by ELISA.Results Compared with those of positive control,the anti-HBs positive conversion rate of CpG-ODN+HB DNA vaccine group increased by 3 folds,and anti-HBs level increased by 2 folds.Compared with those of CpG-ODN+HB DNA vaccine group,the anti-HBs of the four CpG-ODN+recombinant HB vaccine groups converted early,and the anti-HBs level and positive conversion rate induced by recombinant HB vaccine,even at one-third of the original dosage,showed no significant change.Conclusion CpG-ODN improved the immune effects of both HB DNA and recombinant HB vaccines.

关 键 词：CPG-ODN 核酸疫苗 重组疫苗 免疫效果 乙肝 BALB/c小鼠 寡聚脱氧核苷酸 ELISA检测 阳性对照 阴性对照 抗体水平 CHO细胞 联合免疫 生理盐水 疫苗用量 抗体产生 注射 实验 时间 配伍 

分 类 号：S859.797[农业科学—临床兽医学] S852.5[农业科学—兽医学]