W_(135)和Y群脑膜炎球菌培养和多糖纯化  

Culture of Meningococcus Serogroups W_(135) and Y and Purification of Polysaccharide

作　　者：李文春[1] 戴晓畅[1] 杨美峰[1] 钱雯 施競 陈兰萍 陈燕 黄镇 

机构地区：[1]云南大学药学院,昆明650091 [2]云南沃森生物技术有限公司,昆明650118

出　　处：《中国生物制品学杂志》2005年第3期236-237,243,共3页Chinese Journal of Biologicals

摘　　要：目的培养W135和Y群脑膜炎球菌及荚膜多糖的纯化。方法采用综合培养基代替半综合培养基在75L生物反应器中对两群脑膜炎球菌进行培养,研究培养基处方、发酵、多糖提取及纯化工艺。结果综合培养基可代替半综合培养基对两群脑膜炎球菌进行培养。在培养过程中补加葡萄糖溶液、优化搅拌速度及通气量均有利于两菌群生长;在多糖纯化过程中,通过对比试验选择25%乙醇沉淀核酸,2～8℃的酚溶液,萃取蛋白的次数控制在3～4次为好。结论所研制的综合培养基及多糖纯化的工艺具有较好的可行性,为四价脑膜炎球菌多糖疫苗的研制提供了试验基础。Objective To culture meningococcus serogroups W_~135 and Y and purify capsular polysaccharide.Methods Meningococcus serogroups W_~135 and Y were cultured in a 75 L bioreactor,and the formula of medium as well as procedures of fermentation and extraction and purification of polysaccharide were studied.Results Synthetic medium might be used for the culture instead of semi-synthetic medium.Supplying glucose solution and optimizing the stirring rate and airing quantity were beneficial to the growth of meningococcus serogroups W_~135 and Y.During the purification of polysaccharide,25% alcohol might be selected to precipitate the nucleic acid.The times of extraction of protein with phenol solution at 2-8℃ should be controlled at 3-4.Conclusion The developed synthetic medium and purification procedure were feasible and provided an experimental basis for the preparation of tetravalent meningococcus polysaccharide vaccine.

关 键 词：脑膜炎 球菌 Y群 培养基 生物反应器 葡萄糖溶液 荚膜多糖 纯化工艺 多糖提取 培养过程 搅拌速度 纯化过程 乙醇沉淀 试验选择 通气量 菌多糖 研制 菌群 

分 类 号：TS252.54[轻工技术与工程—农产品加工及贮藏工程] S947.2[轻工技术与工程—食品科学与工程]