Study the effects of metallic ions on the combination of DNA and histones with molecular combing technique  

作　　者：LIUYuying WANGPengye DOUShuoxing XIEPing WANGWeichi YINHuawei 

机构地区：[1]LaboratoryofSoftMatterPhysics,InstituteofPhysics,ChineseAcademyofSciences,Beijing100080,China

出　　处：《Chinese Science Bulletin》2005年第8期731-737,共7页

基　　金：supported by the National Natural Science Foundation of China(Grant Nos.60025516 and 10334100).

摘　　要：The effects of monovalent (Na+, K+) and diva- lent (Mg2+, Ca2+, Mn2+) ions on the interaction between DNA and histone are studied using the molecular combing tech- nique. ?-DNA molecules and DNA-histone complexes incu- bated with metal cations (Na+, K+, Mg2+, Ca2+, Mn2+) are stretched on hydrophobic surfaces, and directly observed by fluorescence microscopy. The results indicate that when these cations are added into the DNA solution, the fluorescence intensities of the stained DNA are reduced differently. The monovalent cations (Na+, K+) inhibit binding of histone to DNA. The divalent cations (Mg2+, Ca2+, Mn2+) enhance sig- nificantly the binding of histone to DNA and the binding of the DNA-histone complex to the hydrophobic surface. Mn2+ also induces condensation and aggregation of the DNA- his- tone complex.The effects of monovalent (Na+, K+) and divalent (Mg2+, Ca2+, Mn2+) ions on the interaction between DNA and histone are studied using the molecular combing technique. X-DNA molecules and DNA-histone complexes incubated with metal cations (Na+, K+, Mg2+, Ca2+, Mn2+) are stretched on hydrophobic surfaces, and directly observed by fluorescence microscopy. The results indicate that when these cations are added into the DNA solution, the fluorescence intensities of the stained DNA are reduced differently. The monovalent cations (Na+, K+) inhibit binding of histone to DNA. The divalent cations (Mg2+, Ca2+, Mn2+) enhance significantly the binding of histone to DNA and the binding of the DNA-histone complex to the hydrophobic surface. Mn2+ also induces condensation and aggregation of the DNA-histone complex.

关 键 词：DNA 组蛋白 金属离子 荧光显微镜法 

分 类 号：Q523[生物学—生物化学]