大鼠变应性鼻炎模型下呼吸道细胞因子及黏蛋白的改变  

Alteration of cytokines and mucin in lower respiratory tract in allergic rhinitis model in rats

作　　者：韩菲[1] 安云芳[1] 赵长青[1] 

机构地区：[1]山西医科大学附属第二医院耳鼻咽喉科

出　　处：《中华耳鼻咽喉头颈外科杂志》2005年第5期339-342,共4页Chinese Journal of Otorhinolaryngology Head and Neck Surgery

基　　金：国家自然科学基金(30350006);山西省归国人员基金(00103544);山西省科委自然基金(972187)

摘　　要：目的　探讨大鼠变应性鼻炎模型上、下呼吸道炎症反应的相关性及其机制。方法　选健康大鼠60只,随机分为变应性鼻炎组和对照组,每组30只。以卵清蛋白+Al(OH)3 基础致敏、局部以2%卵清蛋白激发制成大鼠变应性鼻炎模型。取实验动物鼻黏膜及肺组织做HE染色、阿森过碘酸雪夫(alcin blueandperiodicacid schiff, AB PAS)染色,分别观察炎症细胞及杯状细胞;透射电镜观察支气管黏膜上皮变化;以酶联免疫吸附测定法(enzyme linkedimmunosorbentassay, ELISA)检测支气管肺泡灌洗液(bronchialalveoluslavagefluid, BALF)中白细胞介素4 -(interleukin 4, IL- 4 )的水平;以免疫组化方法检测肺组织细胞间黏附分子1 (intercellularadhesionmolecule 1, ICAM -1)及鼻黏膜和肺组织黏蛋白5AC(Mucin5AC,MUC5AC)的表达。结果　大鼠变应性鼻炎模型组鼻黏膜及肺组织有明显的炎症细胞浸润,杯状细胞增生活跃并呈分泌旺盛状态,电镜下支气管黏膜部分纤毛破坏。模型组BALF中IL 4含量( x±s,下同)为(58 .10±7 .92)pg/ml,对照组为(24. 66±2 .07)pg/ml,两组比较差异有统计学意义(t=7. 06, P<0.05),且模型组肺组织ICAM 1及MUC5AC的表达(分别为0. 66±0. 24、0 .71±0 .10)与对照组(分别为0 .23±0 .02、0 .29±0. 03)比较,差异均有统计?Objective To investigate the relationship between allergic rhinitis (AR) and asthma as well as the mechanisms related with it. Methods Sixty healthy rats were randomly divided into AR group and control group. AR model was established by intraperitoneal injection of ovalbumin (OVA) and nasal challenge with OVA. Nasal mucosa and lung tissue from both groups were stained with hematoxylin-eosin (HE) , alcin-blue and periodic acid-schiff (AB-PAS), respectively. At the same time, the lung tissue was studied by transmission electron microscopy (TEM). Enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry were used to examine the level of interleukin-4 (IL-4) in bronchial alveolus lavage fluid (BALF) and the expression of intercellular adhesion molecule-1 (ICAM-1) and MUC5AC in nasal and lung tissue, respectively. Results Infiltration of inflammatory cells in nasal mucosa and lung tissue of AR model in rat was evident. Cilia destruction of bronchial epithelial cells of AR model was found. The level of IL-4 in BALF of AR group (58.10±7.92) pg/ml was significant higher compared with that in control group (24.66±2.07) pg/ml. The expression of ICAM-1 (0.66±0.24) and MUC5AC (0.71±0.10) in lung tissue were both significantly higher than that in control group (0.23±0.02、0.29±0.03). Conclusions Allergic inflammation in nasal mucosa not only leads to changes in both histopathology and immunology, but also initiates the inflammation in lower respiratory tract mainly causing the change of cytokines and mucin.

关 键 词：大鼠 变应性鼻炎 动物模型 下呼吸道细胞因子 黏蛋白 鼻黏膜 

分 类 号：R765.21[医药卫生—耳鼻咽喉科]