转化生长因子β1及其受体与体外受精-胚胎移植后流产关系的研究  

Study on the correlation of transforming growth factor β1 and its receptors with spontaneous abortion after in vitro fertilization and embryo transfer

作　　者：程玲慧[1] 曹云霞[1] 

机构地区：[1]安徽医科大学第一附属医院妇产科,合肥230022

出　　处：《中华妇产科杂志》2005年第5期299-301,共3页Chinese Journal of Obstetrics and Gynecology

摘　　要：目的　了解转化生长因子β1(TGF- β1)及其受体(TβR)在绒毛及蜕膜组织中的表达,初步探讨TGF- β1与体外受精胚胎移植(IVF- ET)后流产及自然流产的关系。方法　应用免疫组化链霉菌抗生物素蛋白过氧化物酶(SP)连接法,检测10例IVF -ET后流产患者(IVF- ET组)和20例早期自然流产患者(自然流产组)的绒毛及蜕膜组织中的TGF -β1及TβR(Ⅰ、Ⅱ型)表达情况,其表达水平以阳性染色部位的吸光度(A)值表示,并与20例人工流产者(对照组)进行比较。结果　TGF- β1及TβR在早孕绒毛滋养细胞、蜕膜组织腺体及间质细胞中均有阳性染色, 3组表达部位相同。TGF β1在IVF ET组蜕膜及绒毛组织中表达水平(A值,中位数,下同)分别为0 167、0 .199,自然流产组为0 .198、0 .201,对照组为0 .277、0 .274;TβR Ⅰ在IVF- ET组蜕膜及绒毛组织中表达水平为0 144、0 150,自然流产组为0 .202、0. 201,对照组为0 .238、0 .281;TβR Ⅱ在IVF ET组蜕膜及绒毛组织中的表达水平为0 .199、0 .145,自然流产组为0. 153、0 .156,对照组为0 .300、0 .301。上述指标,IVF -ET、自然流产组分别与对照组比较,差异均有统计学意义(P<0. 01)。结论　TGF -β1及TβR在IVF- ET后流产及自然流产患者绒毛及蜕膜组织中的表达下降,在IVF- ET后流产发病中起重要作用,?Objective To understand the expressions of transforming growth factor β1(TGF-β1) and its receptors(TβR) in villi and decidua during early pregnancy and their effects on early embryo development, and explore the mechanism of spontaneous abortion. Methods By immunohistochemical technique, expressions of TGF-β1 and its receptors were determined in villi and decidua from 10 cases of spontaneous abortion women after in vitro fertilization and embryo transfer (IVF-ET group), 20 cases of spontaneous abortion women (spontaneous abortion group), and 20 cases of normal early pregnancy women (control group). Results TGF-β1 and its receptors had high expressions in decidua cells, villus glands and interstitial cytoplasm in all three groups. The average light density of the expressions of TGF-β1 in the villi and decidua of IVF-ET, spontaneous abortion and control groups were 0.167 and 0.199, 0.198 and 0.201, 0.277 and 0.274, respectively. The intensity of TβR-Ⅰ in the villi and decidua of IVF-ET, spontaneous abortion and control groups were 0.144 and 0.150, 0.202 and 0.201, 0.238 and 0.281, respectively. And the expressions of TβR-Ⅱ in the villi and decidua of IVF-ET, spontaneous abortion and control groups were 0.199 and 0.145, 0.153 and 0.156, 0.300 and 0.301, respectively. The differences between the control group and both abortion groups were all significant (P<0.01). Conclusions The expressions of TGF-β1 and its receptors in the early pregnancy tissues of aborted women declined. It demonstrates that the abnormal expressions of TGF-β1 and its receptors play an important role in spontaneous abortion, they are one of the causes of spontaneous abortion.

关 键 词：转化生长因子β1(TGF-β1) 受体 IVF-ET 移植后 体外受精-胚胎移植 早孕绒毛滋养细胞 蜕膜组织 抗生物素蛋白 早期自然流产 绒毛组织 阳性染色 过氧化物酶 对照组 TβR-Ⅰ TβR-Ⅱ 初步探讨 免疫组化 表达情况 人工流产 

分 类 号：R711.6[医药卫生—妇产科学]