宫内窘迫新生儿脐动脉血超氧化物歧化酶及丙二醛检测的临床意义  

Clinical values of superoxide dismutase and malondialdehyde detection in cord blood of newborns with fetal distress

作　　者：秦丽娜[1] 冯爱华[1] 杨传华[1] 邢海燕[1] 

机构地区：[1]济南市第四人民医院妇产科,250031

出　　处：《中华妇产科杂志》2005年第5期312-314,共3页Chinese Journal of Obstetrics and Gynecology

摘　　要：目的探讨脂质过氧化与胎儿窘迫,以及与新生儿缺血缺氧性脑病(HIE)的关系.方法将产程中发生宫内窘迫的新生儿60例作为研究对象,其中出生后无窒息者39例为检测Ⅰ组,有窒息者21例为检测Ⅱ组;取同期正常新生儿30例为对照组.检测各组新生儿脐动脉血超氧化物歧化酶(SOD)、丙二醛水平,观察各组新生儿HIE发生情况;分析宫内窘迫持续不同时间的新生儿SOD、丙二醛的变化及HIE发生情况.结果 (1) SOD水平检测:检测Ⅰ组为(12 896±247)U/g Hb,检测Ⅱ组为(9846±268)U/g Hb ,对照组为(17 282±134)U/g Hb.检测Ⅰ、Ⅱ组SOD水平分别与对照组比较,均显著降低,差异有统计学意义(P<0.01).检测Ⅰ、Ⅱ组共发生HIE 9例(HIE组),SOD水平为(7486±245)U/g Hb ,未发生HIE 51例(非HIE组),SOD水平为(13 878±257)U/g Hb,两组比较,差异有统计学意义(P<0.01).检测Ⅰ、Ⅱ组宫内窘迫持续时间≤30 min共19例,SOD水平为(17 411±324)U/g Hb,持续时间31～120 min共26例,SOD水平为(12 076±230)U/g Hb,持续时间≥121 min共15例,SOD水平为(9786±249)U/g Hb.(2)丙二醛水平检测:检测Ⅰ组为(6.3±0.4)μmol/L,检测Ⅱ组为(8.6±1.5)μmol/L ,对照组为(4.1±0.5)μmol/L ,检测Ⅰ、Ⅱ组丙二醛水平显著高于对照组,两者比较,差异有统计学意义(P<0.01).HIE组为(10.6±0.6)μmol/L,非HIE组为(5.1±0.8)μmol/L,两组比较,差异有统计学意义(P<0.01).宫内窘迫持续时间≤30 min者,丙二醛水平为(4.2±0.3)μmol/L ,持续时间31～120 min者为(7.5±1.5)μmol/L ,持续时间≥121 min者为(8.9±1.5)μmol/L .(3)HIE组宫内窘迫持续时间≤30 min者为 0例,31～120 min者为3例,≥121 min者为6例.结论机体脂质过氧化水平与胎儿窘迫的发生关系密切,胎儿窘迫可能是导致新生儿HIE发生率明显增高的重要因素.脐动脉血SOD、丙二醛水平的检测可作为预测HIE的早期诊断指标之一.Objective To investigate the relations between intrauterine asphyxia and peroxidation and newborn hypoxic-ischemic encephalopathy(HIE). Methods The levels of superoxide dismutase (SOD) and malondialdehyde (MDA) in cord blood of 60 newborns with intrauterine asphyxia during labor(which was divided into two groups,39 cases with asphyxia in groupⅠ, and 21 cases with asphyxia in groupⅡ),and in 30 newborns without intrauterine asphyxia(control group) were determined. The levels of SOD and MDA in cord blood of newborns with HIE were compared with those in newborns without HIE. The incidence of HIE was estimated simultaneously. Results (1) The levels of SOD were (12 896±247) U/g Hb in groupⅠ, (9846±268) U/g Hb in groupⅡ, (17 282±134) U/g Hb in control group, significantly lower in the former two groups compared with control group, while the level of SOD in group Ⅰ was higher than that in group Ⅱ(P<0.01). There were nine cases with HIE in groupsⅠand Ⅱ(HIE group) , the level of SOD in these cases was (7486±245) U/g Hb. There were 51 cases with non-HIE(non-HIE group), the level of SOD in this group was (13 878±257) U/g Hb. There was significant difference in the level of SOD between HIE and non-HIE groups (P<0.01). Nineteen cases were in <30 min group, and the levels of SOD was (17 411±324) U/g Hb. Twenty-six cases were in 30-120 min group, and the levels of SOD was (12 076±230) U/g Hb. Fifteen cases were in >121 min group, and the levels of SOD was (9786±249) U/g Hb.(2)The levels of MDA were (6.3±0.4) μmol/L in group Ⅰ, (8.6±1.5) μmol/L in group Ⅱ, and (4.1±0.5) μmol/L in control group, significantly higher in the former two groups compared with control group (P<0.01). The levels of MDA were (10.6±0.6) μmol/L in HIE group and (5.1±0.8) μmol/L in non-HIE group, with significant difference between the two groups(P<0.01). The levels of MDA were (4.2±0.3) μmol/L in ≤30 min group, (7.5±1.5) μmol/L in 31-120 min group and (8.9±1.5) μmol/L in ≥121 min group respectively.(3

关 键 词：宫内窘迫 脐动脉血 丙二醛 临床意义 缺血缺氧性脑病(HIE) 超氧化物歧化酶(SOD) mol/L 新生儿HIE 持续时间 脂质过氧化水平 mol/L 胎儿窘迫 早期诊断指标 发生情况 水平检测 对照组 正常新生儿 moL/L 统计学 研究对象 

分 类 号：R722.1[医药卫生—儿科]