血脾屏障形态学的实验研究  

The study on the morphology character of blood-spleen barrier

作　　者：朱安龙[1] 姜洪池[1] 刘连新[1] 朴大勋[1] 潘尚哈[1] 乔海泉[1] 

机构地区：[1]哈尔滨医科大学附属第一医院普外五科,150001

出　　处：《中华外科杂志》2005年第9期591-594,共4页Chinese Journal of Surgery

基　　金：黑龙江省自然科学基金资助项目(D200439)

摘　　要：目的探讨血脾屏障的形态学特征,研究其组成和变化规律,确立血脾屏障的概念。方法选用Wistar大鼠30只,其中10只经尾静脉注射碳粒。取脾脏组织制作病理切片进行HE染色、Foot染色、Masson染色、免疫组织化学染色(CD68、CD34)及透射电镜病理,观察血脾屏障的形态结构特征。结果碳粒在脾脏中围绕血脾屏障形成环状分布。巨噬细胞,血管内皮细胞,胶原纤维及网状组织在血脾屏障中均有特征性分布。结论血脾屏障是位于边缘区,环绕白髓存在的,窦周血管内皮细胞及其基膜、巨噬细胞、网状细胞和网状纤维(网状组织)及胶原纤维组成的生物屏障,它通过细胞间较致密结合的机械屏障作用和巨噬细胞的生物吞噬作用发挥抗原滤过作用,维持白髓的内环境稳态。它随生发中心的形成而逐渐成熟,通透性发生变化。Objective To study the morphology and functional character of blood-spleen barrier(BSB) and establish the concept of BSB. Methods Thirty healthy Wistar rats were studied. Ten rats were injected with 1.5 ml mixed fluid of India ink and physiological saline through the tail vein. Histological changes of the spleen in all animals were observed with light and electron microscopy, including HE, Foot, Masson staining and immunohistochemistry of CD68 and CD34. Results Most of the carbon particles were within the splenic sinuses in marginal zone but not in the white pulp after 6 h. There was a characteristic distribution of the macrophagocytes, vessel endothelial cell, reticular tissue and collagen fiber in the BSB. Conclusions BSB, surrounding the white pulp, is composed of macrophagocytes, marginal-sinus-endothelial cells and their basement membrane, the reticular tissue (reticular cells and reticular fibers) and collagen fibers. The role of BSB is to keep the microenvironment of white pulp stable. It becomes mature while the formation of germinal center of the white pulp. The permeability of BSB changes during its development.

关 键 词：血脾屏障 实验研究 Wistar大鼠 MASSON染色 免疫组织化学染色 血管内皮细胞 巨噬细胞 网状组织 胶原纤维 形态学特征 尾静脉注射 内环境稳态 变化规律 HE染色 病理切片 脾脏组织 电镜病理 结构特征 生物屏障 网状纤维 网状细胞 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]