PTEN负性调控血管紧张素Ⅱ刺激所致心肌细胞肥大  

PTEN negatively regulates angiotensin-Ⅱ induced hypertrophic response of cardiomyocytes

作　　者：于林君[1] 祝善俊[1] 周裔忠[1] 王江[1] 田颖[1] 

机构地区：[1]第三军医大学新桥医院心血管内科,全军心血管内科中心,重庆400037

出　　处：《第三军医大学学报》2005年第10期975-977,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金资助项目 ( 30 370 584 )~~

摘　　要：目的　研究PTEN对血管紧张素Ⅱ(AngⅡ)所致心肌细胞肥大的影响。方法　构建携带PTEN基因的腺病毒载体,感染原代培养的乳鼠心肌细胞,用AngⅡ作为肥大刺激因素,测定ANF、βMHC与心肌细胞大小。结果　对照组与仅携带GFP的腺病毒感染的心肌细胞在AngⅡ作用下,ANF、βMHC表达与细胞直径显著增高,而PTEN过度表达则对上述改变具有抑制作用。结论　PTEN能够负性调控AngⅡ所致的心肌细胞肥大。Objective To study the effects of PTEN on angiotensin-Ⅱ (Ang-Ⅱ) induced cadiomyocytic hypertrophy. Methods Wild type PTEN gene was transferred into the primary generation of cardiomyocytes from neonatal rats through the recombinant adenovirus containing PTEN gene. Then the cardiomyocytes were irritated with Ang-Ⅱ. ANF, MHC and the size of the cardiomyocytes, which served as the index of hypertrophic response, were determined. Results After Ang-Ⅱ stimulation, the level of ANF and MHC and the cardiomyocytic size were significantly increased in the cardiomyecytes with normal expression of PTEN but the hypertrophic response was markedly suppressed in those with high expression of PTEN. Conclusion PTEN can down-regulate the hypertrophic response of cardiomyocytes induced with Ang-Ⅱ.

关 键 词：FTEN 血管紧张素Ⅱ 心肌细胞肥大 腺病毒 

分 类 号：R322.11[医药卫生—人体解剖和组织胚胎学] R977.6[医药卫生—基础医学]