离子对SPE-HPLC法检测生物检材中的百草枯  

Ion-pair Solid-phase Extraction(SPE) and HPLC Analysis of Paraquat in Biological Sample

作　　者：王瑞花[1] 苏少明 秦光明[1] 李绍鹏 柴育芳 刘清[1] 

机构地区：[1]昆明医学院法医学院,云南昆明650031 [2]玉溪市公安局刑科所,云南玉溪653100

出　　处：《法医学杂志》2005年第2期121-123,共3页Journal of Forensic Medicine

摘　　要：目的建立生物检材中百草枯的简便、快速、灵敏、可靠的高效液相色谱分析方法。方法生物检材酶解后,用以十二烷基三甲基溴化铵和十二烷基硫酸钠预处理过的C18固相柱萃取,HPLC/DAD进行分析。结果回收率81%～94%,检出限为1ng·mL-1,线性范围50ng·mL-1～1mg·mL-1,结论此方法适用于中毒生物检材中百草枯的检测。Objective To establish an HPLC method for the determination of Paraquat in biological samples. Methods Paraquat in biological samples was extracted by C18 columns which were pre-treated with cetyl-trimethyl ammonium bromide(CTAB) and soudium dodecyl sulphate(SDS), and analysed by HPLC/DAD. Results The detection limit of the method was 1ng·mL-1,and the average recoveries were 81%～94%. Conclusion The method can be used to analysis of paraquat in biological samples.

关 键 词：生物检材 百草枯 HPLC法 检测 SPE 离子对 HPLC/DAD 十二烷基硫酸钠 色谱分析方法 三甲基溴化铵 高效液相 线性范围 预处理 回收率 检出限 

分 类 号：R991[医药卫生—毒理学] R595.4[医药卫生—药学]