血液和脑脊液中利多卡因的气相色谱-质谱检测研究  

The Study on Identification of Lidocaine in Blood and CSF by GC/MS

作　　者：尉志文[1] 贠克明[1] 张楠[1] 贺文艳[1] 安健康[1] 高丽波[2] 陈跃华[2] 吴燕滨[2] 牛建国[2] 

机构地区：[1]山西医科大学法医学院,山西太原030001 [2]山西医科大学法医学院2002级,本科生山西太原030001

出　　处：《法医学杂志》2005年第2期124-126,共3页Journal of Forensic Medicine

基　　金：山西省自然科学基金资助项目(20031103);山西省科技攻关项目(051100-1);山西医科大学博士生启动基金项目(200302);山西医科大学学生创新基金项目(200436)

摘　　要：目的建立血液和脑脊液中利多卡因的气相色谱-质谱联用定性、定量检测方法。方法血液或脑脊液盐酸酸化后,氢氧化钠碱化(pH=9),乙醚提取,氮气流下挥干,乙醇定容,气相色谱-质谱联用仪分析,选择离子监测模式检测(86,58,72,87),定性、定量检测血液和脑脊液中利多卡因。结果血液和脑脊液中利多卡因的线性范围为1.0～60.0μg·mL-1(r=0.9999),检出限为0.02μg·mL-1(S/N=3),加样回收率为85%～103%,麻醉致死犬血液和脑脊液中检出利多卡因,结果满意。结论该法选择性好,干扰少,灵敏,准确,可用于生物体液中利多卡因的定性和定量检测。Objective To establish a rapid and simple gas chromatographic-mass spectric method for qualitative and quantitative analysis of lidocaine in blood and cerebrospinal fluid(CSF). Methods Following an acidification of HCl , blood or CSF was alkalinized with NaOH (pH=9) and extracted with ether for two times. Evaporated in a water bath and with an air velocity of nitrogen gas, extract was dissolved with ethanol and analyzed by a gas chromatographic-mass spectrum method, lidocaine was analyzed qualitatively and quantitatively by GC/MS (SIM:86,58,72,87). Results Linear range of lidocaine detected in blood or CSF by this method is 1.0-60.0μg·mL-1(r=0.999 9), the minimum detected concentration of lidocaine was 0.02 μg·mL-1(S/N=3),recovery is at 85%-103%. This method was used in the determination of lidocaine in dog model died of the anesthesia with lidocaine. Conclusion This study provided a gas chromatographic-mass spectric analysis for lidocaine in blood and CSF. This method was more selective, little interferefering, more sensitivities and simpler. It could be used in the detection of lidocaine in biological fluids.[

关 键 词：利多卡因 脑脊液 血液 检测研究 气相色谱-质谱联用仪 定量检测方法 加样回收率 氢氧化钠 监测模式 选择离子 线性范围 麻醉致死 生物体液 定性 检出限 

分 类 号：R169.42[医药卫生—公共卫生与预防医学] R446.14