缺血预处理对大鼠移植胰缺血再灌注损伤的保护作用及其与细胞凋亡的相关性  

Effect of ischemic preconditioning on ischemia reperfusion injury of graft after pancreas transplantation and correlations with apoptosis in rat

作　　者：刘小南[1] 霍婷婷[2] 王为忠[1] 陈彩平[3] 管文贤[1] 陈冬利[1] 

机构地区：[1]中国人民解放军第四军医大学西京医院胃肠外科,陕西省西安市710033 [2]中国人民解放军第四军医大学西京医院麻醉科,陕西省西安市710033 [3]中国人民解放军第四军医大学西京医院小儿科,陕西西省安市710033

出　　处：《世界华人消化杂志》2005年第7期871-876,共6页World Chinese Journal of Digestology

摘　　要：目的:探讨缺血预处理对大鼠移植胰缺血再灌注损伤的早期保护作用及其与细胞凋亡的相关性.方法:正常大鼠6只为对照组,糖尿病SD大鼠24只随机分为缺血再灌注组(I/R组,n=6)和缺血预处理组(IPC组,n=18),IPC组又根据不同方法分为3个亚组:IPC1组(缺血5min再灌注5min1次,n=6)、IPC2组(缺血5min再灌注5min2次,n=6)和IPC3组(缺血5min再灌注5min3次,n=6),I/R组和IPC组均行单纯胰腺移植,24只SD大鼠为供体;检测各组再灌注前、后血糖;再灌注后2h血清中TNF-α和NO的含量、移植胰组织中SOD,MPO和MDA含量;用TUNEL法观察移植胰组织细胞凋亡情况,WesternBlot法检测移植胰组织Bax和Bcl-2蛋白表达情况.结果:再灌注后IPC1(14.3±1.1vs12.1±0.9mmol/L.P<0.05)组、IPC2(12.1±0.9vs16.5±1.4mmol/L,P<0.01)和IPC3组(14.7±1.3vs12.1±0.9mmol/L,P<0.05)相对于I/R组血糖低;IPC2组较IPC1组(12.1±0.9vs14.3±1.1mmol/L,P<0.05)和IPC3组(12.1±0.9vs14.7±1.3mmol/L.P<0.05)血糖低.再灌注后IPC1(1.41±0.17vs1.79±0.25kU/L,P<0.05)组、IPC2(1.05±0.16vs1.79±0.25kU/L,P<0.01)和IPC3组(1.43±0.20vs1.79±0.25kU/L,P<0.05)较I/R组血清中TNF-α含量低;IPC2组较IPC1组(1.05±0.16vs1.41±0.17kU/L,P<0.05)和IPC3组(1.05±0.16vs1.43±0.20kU/L,P<0.05)TNF-α含量低.再灌注后IPC1(13.13±2.87vs8.91±1.23μg/L,P<0.05)组、IPC2(18.79±2.39vs8.91±1.23μg/L,p<0.01)和IPC3组(14.36±1.78vs8.91±1.23μg/L,P<0.05)较I/R组血清中NO含量高;IPC2组较IPC1组(18.79±2.39vs13.13±1.87μg/L,P<0.05)和IPC3组(18.79±2.39vs14.36±1.78μg/L,P<0.05)NO含量高.再灌注后IPC1(179.82±19.54vs153.47±17.67mU/g,P<0.05)组、IPC2(213.64±22.97vc153.47±17.67mU/g,P<0.01)和IPC3组(181.68±20.32vs153.47±17.67mU/g,P<0.05)较I/R.组移植胰组织中SOD活性高;IPC2组较IPC1组(213.64±22.97vs179.82±19.54mU/g,P<0.05)和IPC3组(213.64±22.97vs181.68±20,32mU/g.P<0.05)SOD活性高.再灌注后IPC1(0.70±0.26vs0.87±0.31mmol/g,P<0.05)组、IPC2(0.46±0.18vs0.87±0.31mmol/g,P<0.01)和IPC3组(0.67±0.15vs0.87±0.31mmol/AIM: To investigate the effect of ischemic preconditioning on ischemia reperfusion injury of the pancreas graft in rat, and to analyze the possible mechanism. METHODS: Six normal SD rats in the control group received sham operation. Twenty-four SD rats with steptozozin-in-duced diabetes were randomly assigned to 2 groups: Group I/R consisted of 6 diabetic rats which received pancreas transplantation; Group IPC consisted of 18 diabetic rats which received pancreas transplantation and were exposed to 5 min ischemia and 5 min reperfusion once (IPC1, n = 6), twice (IPC2, n = 6) or thrice (IPC3, n = 6) before ablating donors. Blood glucose, serum NO and TNF-α, MDA, SOD, MPO, TUNEL cells, and the expression of Bcl-2 and Bax pretien (Western Blot) in graft were monitored. RESULTS: The mean blood glucose levels in Group IPC1 (14.3±1.1 vs 12.1±0.9 mmol/L,P<0.05), Group IPC2(12.1±0.9 vs 16.5±1.4 mmol/L, P<0.01) and Group IPC3(14.7±1.3 vs 12.1±0.9 mmol/L, P<0.05) were lower than that in Group I/R, and the glucose level in Group IPC2 was lower than those in IPC1(12.1±0.9 vs 14.3±1.1 mmol/L, P<0.05)and IPC3 (12.1±0.9 vs 14.7±1.3 mmol/L, P<0.05) 2 hours after reperfusion. The mean NO (13.13±2.87 vs 8.91±1.23 μg/L, P<0.05,18.79±2.39 vs 8.91±1.23 μg/L, P<0.01,14.36±1.78 vs 8.91 ±1.23 μg/L, P<0.05) and SOD (179.82±19.54 vs 153.47±17.67 mU/g, P<0.05,213.64±22.97 vs 153.47±17.67 mU/g, P<0.01,181.68±20.32 vs 153.47±17.67 mU/g,P<0.05) levels in Group IPC1, IPC2and IPC3were higher than that in Group I/R, and the levels in Group IPC2 were higher than those in Group IPC1 and Group IPC3 2 hours after reperfusion (18.79±2.39 vs 13.13±2.87 μg/L, 18.79±2.39 vs 14.36±1.78 μg/L, 213.64±22.97 vs 179.82±19.54 mU/g, 213.64±22.97 vs 181.68±20.32 mU/g, P<0.05). The mean levels of TNF-α(1.41±0.17 vs 1.79±0.25 kU/L, P<0.05, 1.05±0.16 vs 1.79±0.25 kU/L,P<0.01, 1.43±0.20 vs 1.79±0.25 kU/L,P<0.05, MDA (0.70±0.26 vs 0.87±0.31 mmol/g, P<0.050.46±0.18 vs0.87±0.31 mmol/g,P<0.01; 0.67±0.15 vs 0.87±0.31 mmol/g

关 键 词：缺血再灌注损伤 缺血预处理 细胞凋亡 移植胰 相关性 Bcl-2蛋白表达 mol/L TNF-α 早期保护作用 MDA含量 Bc1-2蛋白 Bax蛋白表达 SOD活性 MPO活性 单纯胰腺移植 TUNEL法 Westem I/R 胰组织 SD大鼠 NO含量 PMNs A/g 内源性NO 

分 类 号：R657.5[医药卫生—外科学]