人发角蛋白桥接周围神经缺损后的相容性和降解性的实验研究  

Experimental study on the compatibility and degradation of human hair keratin bridging for peripheral nerve defects

作　　者：武雷[1] 罗艳芬[2] 刘晓静[1] 杨俊[1] 陆云涛[1] 肖应庆[3] 秦建强[1] 

机构地区：[1]南方医科大学临床解剖学研究所,广东省组织构建与检测重点实验室,广州市510515 [2]解放军第九十四医院妇儿科,南昌市330002 [3]南方医科大学生化教研室,广州市510515

出　　处：《中华创伤骨科杂志》2005年第5期452-455,共4页Chinese Journal of Orthopaedic Trauma

基　　金：广东科技计划专项(2003A3020101);广东省重点实验室(2001B60107)

摘　　要：目的探讨人发角蛋白(H H K)在桥接周围神经缺损后,诱导神经再生及与周围组织的相容和自身的降解情况。方法用聚乳酸-羟基乙酸(P LG A)套管装配H H K来桥接SD大鼠10m m长的坐骨神经缺损,术后第3、6、9、12周取材,切取中段行H E染色,并对第6周标本加行S100蛋白和神经丝(N F)免疫组化染色,12周标本横切加行透射电镜检查,做组织学观察。结果术后3周H H K毛小皮已经开始剥脱降解,并已有大量的雪旺细胞和神经纤维沿H H K长入,6周时H H K均已质化,免疫组化染色见大量的S100蛋白阳性细胞和N F阳性纤维;9、12周H H K继续降解,长入其间的雪旺细胞和神经纤维更多,排列更加规律,电镜下见已形成较厚的髓鞘板层。结论H H K具有很好的生物相容性和可控的降解速率,能很好的诱导神经再生,可作为支架材料应用于周围神经组织工程研究。Objective To investigate the compatibility and degradation of huma n hair keratin(HHK) bridging for peripheral nerve defects and regeneration of th e peripheral nerve (PN) induced by HHK. Methods The sciatic nerve of SD rats wer e transected with a 10mm gap,then repaired by the polyactic-polylycolic acid ( PLGA) tube with HHK. At different time intervals(3rd,6th, 9th and 12th week)afte r operation, the histological examinations(HE staining), immunohistochemistry(an ti-S100, NF) and transmission electron microscope were used to explore the morp hological changes. Results Large numbers of Schwann cells and nerve fibers regen erating along HHK were observed under optical microscope, and the hair cuticle o f HHK was denudated and degraded at the 3rd week. After 6 weeks, there were plen ty of S100 positive cells and NF positive fibers, and homogenization of HHK occu rred. The degradation of HHK went on, and more and more Schwann cells and nerve fibers with remarkable polarity grew into the graft at 9th and 12th weeks. Espec ially, distinct myelination was observed under transmission electron microscope. Conclusion With controllable degradation rate and good biocompatibility, HHK ca n induce the PN regeneration and be used as scaffold material in tissue engineer ing research of PN.

关 键 词：人发角蛋白(HHK) 聚乳酸-羟基乙酸(PLGA) 周围神经 

分 类 号：R651.3[医药卫生—外科学]