骨髓源神经干细胞修复大鼠周围神经缺损的实验研究  

Experimental study of using neural stem cells derived from bone marrow to repair rats′ peripheral nervegap

作　　者：杜劲松[1] 王剑[2] 张信英[1] 

机构地区：[1]哈尔滨医科大学附属第一医院骨科,哈尔滨市南岗区邮政街23号150001 [2]浙江医科大学附属第二医院骨科,杭州市310009

出　　处：《中国矫形外科杂志》2005年第10期764-766,共3页Orthopedic Journal of China

摘　　要：目的: 探讨骨髓源神经干细胞用于组织工程化人工神经修复大鼠坐骨神经缺损的治疗效果。方法: 36只Wistar大鼠随机分为3组, 每组12只。A组: 将骨髓源神经干细胞与ECM凝胶混合, 种植于几丁糖神经导管中修复10mm坐骨神经缺损; B组: 仅将ECM凝胶种植于神经导管中; C组: 坐骨神经切下10mm, 翻转180°后缝合。16周后, 行大体观察、神经电生理检测、腓肠肌湿重测定、组织学染色, 免疫组化染色和轴突计数等检查。结果: A组的各项检测指标与C组相近, 明显优于B组, 差异显著(P<0. 05)。结论: 骨髓源神经干细胞可作为周围神经组织工程的种子细胞修复周围神经缺损。Objective: To evaluate the effectiveness of using neural stem cells derived from bone marrow to repair a 10mm sciatic nerve gap.Method: Thirty six Wistar male rats were randomly divided into three groups.group A: Chiposan tubes were seeded by neural stem cells derived from bone marrow together with ECM gel.group B: Chiposan tubes were Seeded only with ECM get.group C: autografts.Sixteen weeks later,a series of examinations were performed which included morphological,electrophysiological、histological staining and immunostaining of S100 and Brdu、 axon counts.Result: Sixteen weeks after the operations,all results of examination of A and C groups were better than B groups( P < 0.05 ).Conclusion: Neural stem cells derived from bone marrow as seeding cells for peripheral nerve tissue engineering can be used to repair nerve gap.

关 键 词：骨髓干细胞 神经干细胞 组织工程 

分 类 号：R651.3[医药卫生—外科学]