利用分子标记辅助选择聚合水稻基因Xa21和Pi9(t)  

Pyramiding Xa21 and Pi9(t) in Rice by Marker-Assisted Selection

作　　者：倪大虎[1] 易成新[2] 李莉[2] 汪秀峰[2] 王文相[3] 杨剑波[2] 

机构地区：[1]安徽农业大学农业学院,合肥230036 [2]安徽省农业科学院水稻研究所,合肥230031 [3]安徽省农业科学院植物保护研究所,合肥230031

出　　处：《分子植物育种》2005年第3期329-334,共6页Molecular Plant Breeding

基　　金：国家863计划(2004AA211163);国家转基因植物研究与产业化开发专项(JY03-B-11);安徽省自然科学基金(03041102)项目资助。

摘　　要：通过有性杂交和田间多代选育,利用分子标记辅助选择和田间/温室抗性鉴定,将广谱高抗白叶枯病的Xa21基因和高抗稻瘟病的Pi9(t)基因聚合到同一品系中,获得双基因纯合且农艺性状稳定的株系。用中国7个和安徽省流行白叶枯病病菌以及多个稻瘟病小种进行抗病性鉴定,结果表明:双抗基因系同时抗白叶枯病和稻瘟病,抗性级别为HR-R,抗谱与供体亲本一致,抗性水平基本相当。室内考种结果显示:双抗基因系间株高变异较大;单株有效穗比两亲本弱;结实率和千粒重明显优于Xa21基因的供体M12,与Pi9(t)基因的供体亲本75-1-127相当。The homozygous lines which conferred the broad spectrum and high resist ance genes Xa21 and Pi9(t) and had stable agronomic characters were obtained by marker-assisted selection (MAS), successively agronomic selection and inoculatio n of pathogen Xoo and blast in the field /greenhouse. According to the performan ce of artificial inoculation of multiple strains in the field the lines expresse d high resistance to bacterial blight and blast and had same spectrum and simila r resistant level as donor varieties. The assay of agronomic characters displaye d that the plant height varied greatly and the productive tiller numbers per pla nt were lower than donor parents. But fertility and 1 000 grains weight were bet ter than M12 (donor of Xa21) and similar with 75-1-127 (donor of Pi9(t)).

关 键 词：分子标记辅助选择 XA21 水稻基因 高抗白叶枯病 抗病性鉴定 有性杂交 抗性鉴定 基因聚合 抗稻瘟病 农艺性状 抗性水平 室内考种 基因系 安徽省 亲本 供体 有效穗 千粒重 结实率 M12 田间 选育 温室 株系 纯合 小种 病菌 

分 类 号：S511[农业科学—作物学] S511.03